We are proud that we are launching “Have 4,000 Students sign a Jeju 4.3 Petition to the U.S. Capitol” for enactment of “the Korea Jeju 4.3 Human Rights Act 2022” in 2022, which was result of various visits to offices of U.S. Congressman Mark Takano and Congresswoman Judy Chu on May 1, 2019. It was historic sentence for Jeju 4.3 Survivors, Mr. Dong-Soo Park of Sogil Village, Mr. Wonhue Boo of Hwabuk village, Ms. Hee-Chun Oh of Harye village dismissed all charges against the accused on January 17, 2019 by Presiding Judge Jaegal, Chang, Chung, Seung-jin and Seo, Youngwoo at the Korea Jeju Distict Court. “South Korean victims who were wrongfully charged with insurrection during an anti-communist crackdown from 1948 to 1949 and thrown in jail for defying violent paramilitary and military forces, are to receive more that $4 million in compensation for wrongful imprisonment following the Jeju 4.3 massacre, “South Korea Jeju Massacre victims awarded $ 4M in damages,” by Elizabeth Shim: UPI, August 21, 2019).
In this study, we attempted to solubilize protein in slaughter blood (SB) using ultrasonic technology. The application of ultrasonic technology can make enzymatic degradation of SB more effective, which has no comparable alternative for treatment. The SB was homogenized by grinding it for 10 minutes at 10,000 rpm as a pretreatment for preventing its clotting, and then ultrasonic treatment was attempted to solubilize protein in SB. To maximize the efficiency of ultrasonic treatment for SB, the optimum condition of ultrasonic frequency (UF) was determined to be 20 kHz. To optimize the operation conditions of ultrasonification with 20 kHz of frequency, we used response surface methodology (RSM) based on ultrasonic density (UD) and ultrasonification time (UT). The solubilization rate (SR) of protein (%) was calculated to be 101.304 - 19.4205 X1 + 0.0398X2 + 7.9411X12 + 0.0001X22 + 0.0455X1X2. From the results of the RSM study, the optimum conditions of UD and UT were determined at 0.5 W/mL and 22 minutes, respectively, and SB treated under these conditions was estimated to have a 95% SR. Also, experimentally, a 95.53% SR was observed under same conditions, accurately reflecting the theoretical prediction of 95%.