In rice, the stage of the meiosis in the pollen is sensitive stage resulted in the pollen sterility to reduce yield. Dianxi4 is a cold tolerant line. To monitoring the proteome expression patterns in the pollen of Dianxi4 under the cold stress, shotgun proteomic analysis was conducted to the anther of Dianxi4. The rice plant was grown in the peedy rice field then in the 10 DBH(days before heading), one individual rice plant was moved in the growth chamber under the condition of12℃/RH70%(12h day/12h night). Also the plant used as control was moved in the growth chamber unde the condition of 28℃/RH70%(12h day/12h night). after 4 days treatment, the plant were moved in a greenhouse. The treated rice anther were collected in the one day before heading. From the shotgun proteomic analysis, total of 3,855 non-redundant proteins were identified. Among them, 2,360 proteins were reproducibly identified through the treatment and replications. By the T-test, 1,181 differentially expressed proteins were detected. Through the GO analysis, proteins related in gene expression, cellular process, cellular biosynthetic process were enriched.