This study was conducted to evaluate the efficacy of mulberry leaf mixed diet on larval growth of Protaetia brevitarsis. As a result of adding mulberry leaves to oak fermented sawdust, the survival rate of P. brevitarsis larvae were higher than those of the control when mass rearing larvae. When fed oak fermented sawdust with 5%, 10% and 20% of mulberry leaves, the cumulative proportion of larvae over 2.5g was 93.1% at 65 days, 95.6% at 55 days, and 93.9% at 55 days after rearing at 25±1℃, respectively. Also, heavy metals were not detected in larvae of P. brevitarsis fed on mulberry leaves.

This study was conducted to evaluate the efficacy of mulberry cake mixed diet on larval growth of Protaetia brevitarsis. As a result of adding mulberry cake to oak fermented sawdust and mulberry fermented sawdust, the survival rate of P. brevitarsis larvae were higher than those of the control when mass rearing larvae. When fed oak fermented sawdust with 3, 5% and 10% of mulberry cake, the cumulative proportion of larvae over 2.5g was 92.9% at 65 days, 90.8% at 55 days, and 96.3% at 45 days after rearing at 25±1℃, respectively. When fed mulberry fermented sawdust with 3%, 5% and 10% of mulberry cake, the cumulative proportion of larvae over 2.5g was 86.2% at 85 days, 90.7% at 75 days, and 91.5% at 75 days after rearing at 25±1℃, respectively.

In this study 300 weaned pigs (Landrace × Yorkshire × Duroc, 23±3 d of age, 5.56± 1.21 kg initial body weight) were used to study the effect of fungal (Aspergillus oryzae, FSP-A) and fungal + bacteria (Aspergillus oryzae + Bacillus subtilis, FSP-B) fermented soya proteins on their blood hematology, enzymes and immune cell populations. Pigs were allotted to 5 treatments, each comprising of 4 pens with 15 pigs. Basal diets consisted of 15% soyabean meal (Control diet) while for treatment diets SBM was replaced with 3 and 6% of each FSP-A and FSP-B, respectively. The experimental diets were fed from 0 to 14 day after weaning and then a common commercial diet was fed from 15 to 35 day. Blood was collected on 14 and 35 day of experiment and analyzed for hematology, aspartate transaminase (AST), alanine transaminase (ALT) and immune cell populations. At d 14, lower RBC count, Hb and HCT values and higher AST values were noted in pigs fed FSP-A diets when compared with Control and FSP-B fed pigs.Also at d 14 pigs fed 6% FSP-A had lower NE (P<0.05) when compared with those fed 6% FSP-B. The level of FSP influenced the RDW on d 14 and MCHC, MO and MPV on d 35. In addition on d 35, pigs fed 3% FSP-A had lesser NE than those fed 6% FSP -A and Control diet, while pigs fed 6% FSP-B had the highest number of MO compared to other treatments. But there were no differences in the plasma AST and ALT values on d 35. Thus it may be concluded that the FSP either by fungal or fungal + bacterial sources had an influence on the blood hematological status and the populations of immune cells.

A total of 240 weaned pigs (Landrace ×Yorkshire × Duroc, 22±3 d of age, 5.16±0.90 kg initial body weight) were used to study the effect of feeding level of microbial fermented soya protein on their blood hematology, enzymes and immune cell populations. The microbial (Aspergillus oryzae + Bacillus subtilis) fermented soya protein (FSP) was used. Pigs were allotted to four dietary treatments, each comprising of 4 pens with 15 pigs. Basal diets consisted of 15% soya bean meal (Control diet); while for treatment diets SBM was replaced with 3, 6 and 9% FSP. The experimental diets were fed from 0 to 14 day after weaning and then a common commercial diet was fed from 15 to 35 day. Blood was collected on 14 and 35 day of experiment and analyzed for hematology, plasma aspartate transaminase (AST), plasma alanine transaminase (ALT) and immune cell populations. Increasing the level of FSP in the diet of pigs linearly decreased distribution of red blood cells (P<0.01), MPV (P<0.05), MO (P<0.05), EO (P<0.05) and BA (P<0.05) on d 14. Linear and quadratic decrease in the RBC (P<0.05), Hb (P<0.05), HCT (P<0.01), PLT (P<0.001) and EO (P<0.05) and linear increase in the MCHC (P<0.001), MPV (P<0.05), WBC (P<0.05) and NE (P<0.05) on d 35 was noted. Pigs fed with 6% FSP had lower (P<0.05) levels of AST and ALT on d 14, while the levels of ALT and AST on d 35 did not differ among the dietary treatments. Thus the results suggest that microbial fermented soya protein affected the hematological indices, immune cell populations and plasma enzymes in weaned pigs.