An endemic plant to South Korea, Sedum zokuriense Nakai, has medical and floricultural potential and is of ecological importance. Today, many species under the genus Sedum are used as green-roofing systems, sold as ornamental plants, and studied for breeding programs. As such, optimization studies should be conducted to identify key environmental and cultivation factors that would affect their survival and vegetative growth. In this study, shading levels (50%, 65%, 80%, 95%, and 98%), potting mixes (decomposed granite, fertilizer-amended media, perlite, river sand, burnt husk, and vermiculite), and fertilization rates [(control, 0 ppm), 500, 750, 1,000, and 2,000 ppm] were investigated and the responses of S. zokuriense in terms of their survival rate, plant growth and development, CIELAB color reading, and chlorophyll content under greenhouse conditions. Results showed that these stonecrop species are shade-loving and thrive in low-light conditions. Although the fertilizer use had minimal impacts, growing plants at 65% shading, planted with RS:VL:PL (6:2:2, v/v/v) potting media have substantially produced a high survival rate in propagation using stem cuttings. Furthermore, this allowed plants to be established while supporting high vegetative growth, green and healthy plants with high chlorophyll content.