본 연구에서는 중국산 발효 보이차 잎을 고농축으로 추출하였다. 또한 이 추출물에 대한 항산화 활성 및 항노화 효과를 평가하여, 화장품 산업에 응용하고자 하였다. 발효보이차 추출물의 수득율은 17.9%이었으며, 총 폴리페놀량은 37.5%, 탄닌 7.5%, 다당체류 25%, 기타 22%, 수분 8%가 함유되어 있었다. 발효보이차 추출물의 항산화 (in-vitro)활성은 30mg/mL에서는 33.7±1.8%, 50mg/mL에서는 39.4±2.2%의 소거효과를 보였다. 비교군인 초산토코페롤과 녹차추출물 보다 높은 항산화 효과를 보였다. 발효보이차 추출물의 콜라겐 합성율(in-vitro)은 1 mg/mL에서는 102.9±9.9%, 5 mg/mL에서는 111.5±9.9%, 10 mg/mL에서는 122.7±12.2%, 30 mg/mL에서는 131.5±13.7%의 높은 합성능을 보였다 (*p-value?0.05, n=3). 8시간 후의 피부보습효과(in-vivo)는 비교군보다 38.5%의 높은 효과를 보였다. 이 보습력은 도포전보다 32.7% 상승하였다. 발효보이차 추출물은 화장품산업에서 보습효과를 가진 원료로 응용이 가능할 것으로 기대한다.

Provitamin B5 liquid crystal (PVB5-LC) was the new emulsion system to enhance moisturizing activity on the skin. In this study, it should be mentioned that PVB5-LC could be prepared with the main compound of hydrogenated lecithin (HL) in oil-in water (O/W) emulsion. The key ingredient of humectants was contained 2% of provitamin B5 (PVB5) into the PVB5-LC. The best suitable compositions of PVB5-LC were made from 4.0 wt% of HL, 4.0 wt% of cetostearyl alcohol (CSA) as emulsifier and gelling agent, 3.0 wt% of 1,3-butylene glycol (1,3-BG) and 2.0 wt% of glycerin as moisturizers, 3.0 wt% of cyclomethicone (CMC), 3.0 wt% of isononanoate (ININ), 3.0 wt% of capric/caprylic triglyceride (CCTG), 3.0 wt% of macadamia nut oil (MNO) as emollients. As the analytical result of PVB5-LC, it could know that the distribution range of particle size was 0.14 to 12.37 m level (mean size 3.24 m). It was certified the multi lamellar phase around the droplet of liquid crystal when observed the droplet particles through a polarization microscope. And it clinically was tested the effectiveness of moisturizing activity (in-vivo) compared with control sample (O/W emulsion). The effectiveness of moisturizing activity of PVB5-LC with Skincon-200EX after 6 hours went up 49.0% (p〈0.05, n=20) whereas the effectiveness of moisturizing activity of generally O/W emulsion cream was 25.7% (p〈005, n=20). Also, in case of Comeometer CM-825, the moisturizing activity of PVB5-LC after 6 hours rose 36.6% (p〈0.05, n=20) whereas the effectiveness of moisturizing activity of generally O/W emulsion cream was 10.8%. Therefore, it was known that the effectiveness moisturizing activity of PVB5-LC with HL was remarkably superior compared with O/W emulsion cream.