A novel oxidant fumigation (NOF) is a commercial bleaching and disinfection agent. Recent study indicates its insecticidal activity. However, its exact mode of action to kill insects is not known. This study sets up a hypothesis that reactive oxygen species released from NOF is a main factor to kill insects. Plodia interpunctella is a lepidopteran insect pest infesting various stored grains. Both larvae and adults were susceptive to NOF. To test the hypothesis, we needed to identify antioxidant genes in P. interpunctella. Superoxide dismutase (SOD) and thioredoxin-peroxidase (Trx) were identified from P. interpunctella EST library using ortholog sequences of Bombyx mori. Both SOD and Trx were expressed in larvae of P. interpunctella expecially against oxidative stress induced by bacterial challenge. The bacterial challenge also induced some heat shock protein (HSP) genes. Similarly, different doses of NOF significantly induced both SOD and Trx genes. There results suggest that NOF at sublethal doses releases reactive oxygen species, which may be detoxified by the antioxidant activities of SOD and Trx of P. interpunctella.

Nilaparvata lugens Stål is one of the important migratory pests of rice paddy fields in Korea. Resistance levels to nine insecticides were monitored in 12 local strains and correlation analysis was conducted to determine cross-resistance relationships among the tested insecticides. The local strains revealed 1.3- to 28.0-, 1.6- to 6.0-, 2.8- to 237.0-, 0.6- to 0.9-, and 0.7- to 1.3-fold resistance to carbamates, organophosphates, neonicotinoids, fipronil and etofenprox, respectively. Organophosphates revealed moderate correlations with benzofuranyl methylcarbamate (r = 0.566 - 0.614, p > 0.01). Three neonicotinoids were not correlated each other, but imidacloprid and clothianidin were moderately correlated with several benzofuranyl methylcarbamate and organophosphate insecticides (r = 0.590 - 0.705, p > 0.05), indicating that unknown common factors (such as detoxification enzymes) might contribute to resistance to both insecticides. Fipronil and etofenprox exhibited low levels of resistance and cross-resistance with other insecticides, suggesting their potential as an effective insecticide for field application. Resistance level monitoring and correlation analysis would be valuable for the selection of appropriate insecticides to control insecticide-resistant N. lugenes, a typical migratory pest in Korea.

Symbiotic bacteria are common in insects. Because symbiotic bacteria are known to intimately affect the various aspects of insect host biology, ideally insects can be controlled by manipulating their symbiont. However, the attempts to control insects through their symbiont have been very limited. The paucity of the insect pest control using their symbiont is most likely due to the poor understanding of the symbiotic interactions between host insect and symbiont, which is attributed to the difficulty in cultivation of insect symbionts. However, the recently established bean bug, Riptortus pedestris, symbiotic system provides good opportunities to study insect’s symbiont in molecular level through their cultivable symbionts. Bean bugs acquire genus Burkholderia cells from environment and harbor them as their gut symbionts in the specialized posterior midgut. The genome of the Burkholderia symbiont was sequenced, and the genomic information has been used to generate the genetically manipulated Burkholderia symbiont strains. After orally administering the mutant Burkholderia symbionts into bean bugs for symbiotic association, the bacterial colonization levels in the host gut and host phenotypes were analyzed. As a result, we have identified novel symbiotic factors necessary for establishing successful association with host. Our recent understandings on the bacterial symbiotic factors demonstrate a great possibility to control the bean bug pest using genetically modified Burkholderia symbiont.

아스파라거스(Asparagus officinalis)에서 발생하는 해충의 종류 및 피해를 구명하기 위하여 2007년부터 2010년까지 제주도에서 조사를 수행한 결과, 다음과 같이 총 5개목 16종의 해충이 관찰되었다: 파총채벌레(Thrips tabaci), 하와이총채벌레(Thrips hawaiiensis), 차애모무늬잎말이나방(Adoxophyes honmai), 담배거세미나방(Spodoptera litura), 파밤나방(Spodoptera exigua), 왕담배나방(Helicoverpa armigera), 도둑나방(Mamestra brassicae), 네눈쑥가지나방(Ascotis selenaria), 줄고운가지나방(Ectropis excellens), 선녀벌레(Geisha distinctissima), 목화진딧물(Aphis gossypii), 복숭아혹진딧물(Myzus persicae), 청동풍뎅이(Anomala albopilosa), 아스파라거스잎벌레(Crioceris quatuordecimpunctata), 달팽이(Acusta despecta sieboldiana), 작은뾰족민달팽이(Deroceras reticulatum). 그 중에서 파총채벌레는 아스파라거스 전 기간에 걸쳐 발생하여 피해를 주는 해충이었다. 아스파라거스잎벌레와 달팽이류는 봄 수확기에 아스파라거스 순을 가해하여 큰 피해를 주었다. 아스파라거스 입경기부터 생육기에는 진딧물과 나방류 해충이 발생하여 피해를 주었다. 나방류 해충 중에서 파밤나방, 담배거세미나방, 왕담배나방 등 밤나방과 해충의 피해가 많았다. 본 결과를 바탕으로 아스파라거스 재배에서 해충방제 전략에 대하여 고찰하였다.

Amur silver grass, Miscanthus sacchariflorus is one of the promising biofuel crops. A damage of noctuid pest, Leucapamea askoldis was firstly observed from Amur silver grass in Hwasun silver grass plantation during the survey of insect pests of Amur silver grass in Iksan, Hwasun, and Sancheong plantation areas in Korea. The host of L. askoldis was not known yet in Korea. Thus, M. sacchariflorus was the first known host in Korea. The L. askoldis damage was observed as larval feeding on newly grown shoots of M. sacchariflorus close to soil surface from early April to early May in 2013. Investigated larval density was 1.6 ± 1.1 per m2 on April 4 and damage rate of shoots was 0.8% ± 0.4 per hundred plants on May 4, 2013. The larvae bore into shoots of M. sacchariflorus and then feed inside of plant. The damaged shoots are easily pulled out and distinguished by the boring hole on the shoots.

Materials are limited for pest control and chemical pesticides are prohibited in organic farming. Thus, the concept of pest control may not be effective for pest management in organic farm. As an alternative approach, landscape management is potentially effective and useful approach for preventing pathogen and insect pests in organic farm. It is needed to develop best practices utilizing functional biodiversity for pest management through landscape management. For this purpose, we investigated the effect of companion plants on species richness of insect pests and natural enemies in the field border area and in the crop plants (rice and soybean). In rice paddy fields, Lysimachiae foenum-graecum, Saururus chinensis were selected as effective companion plants on the paddy field levee for conserving Arachnidae as natural enemy and density suppression of insect pest such as Lissorphoptrus oryzophilus, Riptortus clavatus. As a result, damage rate of rice plant by insect pests was significantly lower (2.5%) and yield rate of healthy grains was significantly higher (8.7%) than control. In soybean fields, Marigold plants such as Tagetes patula, T. officinalis, increased Arthropod species richness and ratio of natural enemy group, and decreased ratio of insect pest group. As a result, yield (weight of 100 seeds) of healthy soybean was significantly increased (2.5%) than control. The 2nd infection rate from damage by R. clavatus was significantly lower in the treated plot than that in control. Thus, yield rates of healthy soybean in the control and the companion plant-treated plot were 79.0% and 84.3%, respectively, compared to conventional farming using chemical pesticides. Therefore, use of companion plants on field border was an effective practices for preventing pest insects and increasing yield in organic farming.

Grapevines are cultivated globally, and are also damaged by a large number of insect pests especially including leafhoppers. Leafhoppers are one of the important insect pests on various crops including grape. Among them, the genus Arboridia Zachvatkin (Hemiptera: Auchenorrhyncha: Cicadellidae: Typhlocybinae) was known as insect pests against grape farming. Eighty species were described in the genus in the world, and eight species were recorded in the Korean Peninsula so far, which are: Arboridia apicalis, A. kakogawana, A. koreacola, A. maculifrons, A. nigrigena, A. okamotonis, A. silvarum, A. suzukii. Among them, A. apicalis, A. kakogawana, A. maculifrons, A. nigrigena, A. suzukii have been known as important insect pests on grapes. In this talk, we propose a new species from the Korean Peninsula, and confirmed two new records: A. agrillacea and A. lunula. Additionally we present differential diagnoses of the Arboridia species damaging on grapevines for the field of applied biology.

Bacillus thuringiensis (Bt) is a Gram-positive and soil-dwelling bacterium and well known for its ability to produce insecticidal parasporal crystalline protein inclusions, which have attracted worldwide interest for effective pest management. A diseased silkworm by Bt was first discovered by Japanese scientist Ishiwata Shigetane in 1901, and in 10 years, it was re-discovered in Germany by Ernst Berliner, who isolated it as the cause of a disease called Schlaffsucht in flour moth caterpillars. The first commercial product, Bt. kurstaki HD-1 was released to a market in France in 1938. Optimization of mass production for crystal production and cost down enabled the industrialization to be successful, and now many products, such as WP, EC, SC and tablets are used worldwide. In 1976 Robert A. Zakharyan found that plasmids in Bt are involved in the production of crystal proteins and endospores. Pore formation model and signal transduction model were revealed to explain the mode of action of Bt. Works on Bt resistance included a group of receptors of crystal proteins, such as cadherin, APN and ALP. In 1996, a Bt cry gene was integrated to cotton, which successfully reached markets. AtMT technology was used to generate Bt crops. Now the area planted worldwide to genetically engineered Bt crops increased to 66 million hectares. Refuge may be particularly important in slowing the spread of insects resistant to the Bt insecticides. Researchers are trying to increase the insecticidal efficacy of integrated Bt crystal proteins using recent biotechnology.

본 연구에서는 과수의 형태, 거리에 따라 약대가 항상 적정 살포 거리를 유지하며 작업을 진행할 수 있도록 초음파 센서의 신호를 실시간으로 받아 약대를 제어하였고, 또한 약대의 보호를 위하여 진행 방향에 장애물이 존재할 경우 회피할 수 있도록 프로그래밍 하였다. 제작된 시스템으로 현장에서 비 자동제어 상태와 자동제어 상태로 실험을 수행하였다. 실험은 과수의 기둥과 잎 부위에 감수지를 일정간격으로 설치하고 시스템을 이용하여 분사한 후 감수지의 영상을 스캔하여 영상처리를 통해 분석하는 방법으로 이루어졌다. 상 방향 분사 실험에서는 시스템과 대상의 거리가 0.9m~1.1m로 설정해둔 적정거리를 벗어나지 않았기 때문에 비 자동제어와 자동제어 상태 모두 양호한 결과를 보였다. 하지만 측 방향 분 사 실험에서는 비 자동제어 시 우측 열은 98.09%의 분 사율을 보였으나 좌측 열은 69.25%로 낮게 나타났다. 이는 실험이 수행된 배 과원의 경우 과수의 좌측 열이 수평하게 식재되어 있지 않았기 때문으로 비자동제어 상태에서는 좌측열의 과수에 분사되는 양이 줄어들었으나 자동제어 상태에서는 좌, 우측열의 과수에 분사되는 양이 각각 92.66%, 94.64%로 균일하게 나타났다. 시스템 의 제어 속도를 측정하기 위하여 방향 별 약대의 속도를 측정하였고 각각의 속도는 수직방향 100mm/s, 수평 방향 100mm/s, 각 변화 3o/s로 측정되었다. 초기 목적했 던 바와 같이 과수의 형태, 거리에 따라 약대가 적정 거리를 유지하며 작업을 진행함으로 인해서 균일한 살포량을 유지 할 수 있음을 확인하였다.

오이에서 가장 피해가 심한 해충인 목화진딧물과 꽃노랑총채벌레에 방제를 위하여 사용하고 있는 천적인 콜레마니진디벌과 미끌애꽃노린재에 대하여 노균병과 흰가루병 약제의 저독성을 평가하여 살균제와 동시에 활용한 목화진딧물과 꽃노랑총채벌레에 대한 천적의 방제효과를 조사하였다. 47종 살균제 중 DBEDC, hexaconazole, pyraclostrobin, tribasic copper sulfate, triflumizole, chlorothalonil, flusilazole, folpet, carbendazim+diethofencarb, cymoxanil+fenamidone, trifloxystrobin은 콜레마니진디벌 머미에 50% 이상의 독성을 보였고 저독성 살균제 중 azoxystrobin이 노균병과 흰가루병을 동시에 방제할 수 있어 천적과의 동시활용 약제로 선발하였다. 꽃노랑총채벌레와 목화진딧물은 천안과 공주에서 4월 상순 동시에 급격히 밀도가 증가하였고, azoxystrobin 50% 액상수화제를 10일간격으로 계속 방제 하면서 콜레마니진디벌을 진딧물 발생초기 1주간격 3회, 미끌애꽃노린재를 발생초기 1주간격 3회 방사 후 천적 밀도를 조사한 결과 목화진딧물과 꽃노랑총채벌레의 밀도를 억제하면서 콜레마니진디벌 머미는 하엽당 최대 18마리, 미끌애꽃노린재는 꽃당 0.5마리를 나타내어 azoxystrobin이 콜레마니진디벌과 미끌애꽃노린재의 밀도형성에 악영향을 주지 않았다. 시설오이에서 콜레마니진디벌과 미끌애꽃노린재에 안전한 선택약제와 천적을 동시 이용할 경우 효과적인 진딧물과 총채벌레 방제가 가능할 것이다.

The exotic aphid pest, pomegranate aphid, Aphis punicae Parsserini, 1863 (Hemiptera: Aphididae), is newly recognized in the Korean peninsula. This species is originally distributed throughout the Mediterranean regions, the Middle East, Ethiopia, India, Pakistan, Indonesia, and Turkey. In the major producers of pomegranate such as India and the Mediterranean regions, this species is regarded as one of the most serious insect pests inflicting injury on pomegranate, Punica granatum Linnaeus. By sucking plant sap and excreting honeydew, aphids cause various plant diseases and virus infections which are more likely to occur as population size increases. Aphis punicae infests together commonly with Aphis gosspii Glover, and rarely with Aphis craccivora Koch and Aphis spiraecola Patch on P. granatum in Korea. In this study, we first compared two closely related species, A. gossypii and A. punicae, based on morphometric analysis to confirm their morphological relationships. Second molecular identification using mitochondrial COI barcode region was performed for the aphid samples collected from P. granatum.

We previously reported Pear Pest Forecasting Management System (PPFMS) for the Improvement of pass ratio of Korean exporting pears. It is consisted of regular field forecasting by pear farmers, meteorological data obtained by automatic weather station (AWS), an internet web page (http://pearpest.jnu.ac.kr/) as information collecting and providing ground, and information providing service. Currently, we are expanding this system to the area, Cheonan and Ansung, where pear orchards are organized into exportation-specific group. Further, the information obtained from field forecasting and AWS were up-loaded to under-constructing upgraded webpage (http://www.kpear.kr), with several pest/disease-related information. We hope this pest forecasting management system increases the pass ratio of Korean exporting pears throughout establishment of farmer-oriented forecasting, inspiring farmers’ effort for the prevention and forecasting of diseases and pests occurring at pear orchards.

Rapid identification of pest species found under quarantine is an important factor in preventing an economic loss of agricultural commodities. In this study, we analyzed RNA-Seq of the larvae of C. sasakii, G. molesta and G. dimorpha, which are serious pests in several fruits in Korea and are difficult to discriminate by species in their larval stage because of lack of a morphological character. To select immunological diagnostic markers, discriminating the larvae of C. sasakii from the G. molesta and G. dimorpha, RNA-Seq was performed for the larvae of the three insects. The 454 pyrosequencing generated 3,058-4,686 contigs for each three pest species, which assembled into 2,584-3,970 isotigs with average lengths of 829-1,244 bp. Functional annotation of the sequencing results generated 774 orthologs for the three pest species, and 12 isotigs were finally registered as candidate markers for species discrimination through bioinformatical screening, literature search, and gene expression study. The selected candidates include serine proteases, serpins, 27 kDa glycoprotein and storage protein with a constitutive gene expression in their larvae, pupae and adult stage.

Riptortus pedestris (F.) inflict losses on a wide range of crop plants. Biological control is an alternative approach to reduce the bean bug population. Scelionid Gryon japonicum (Ashmead) and Encyrtid Ooencyrtus nezarae Ishii are the major egg parasitoids of R. pedestris. The aggregation pheromone of R. pedestris serves as a kairomone to its egg parasitoids. A new tool was developed using aggregation pheromone in a trap which attracts both con-specific bugs and the parasitoids. Non viable host eggs were added with the trap so as to provide resource for the attracted parastioids to multiply. Several studies were conducted to develop this new tool for trapping of the bugs and enhancing parasitism simultaneously. Aggregation pheromone trap added with host eggs was found to increase temporal parasitism in field by 44% compared to the parasitism without host eggs. Higher parasitism (up to 91%) was found in the sites where aggregation pheromone trap added with host eggs was installed compared to control sites. In order to improve this tool, a new device with twelve sections to hold eggs and to be attached to the pheromone trap was designed to provide maximum exposure of the eggs to the parasitoids and to minimize the competition among the parasitoids. Parasitism and number of female R. pedestris trapped in new device attached trap were higher compared to the conventional. With better performance in trapping R. pedestris and enhancing parasitism, the tool would serve as an IPM technique that targets killing the pest and enhancing activity of the pest’s natural enemy simultaneously.

제주도에서 열대과수로 망고(mango; Mangefera indica), 용과(dragon fruit; Hylocereus undatus), 아떼모야(atemoya; Annona cherimola× A. squamosa) 등이 주로 재배되고 있다. 2008년부터 2011년까지 이들 작물에 발생하는 해충 종류와 피해를 조사하였다. 망고 해충 중 총채벌레, 진딧물, 깍지벌레, 나방류 피해가 많았다. 오이총채벌레(Thrips palmi)와 볼록총채벌레(Scirtothrips dorsalis)는 망고 전 재배기간에 걸쳐 발생하여 큰 피해를 주었다. 오이총채벌레는 주로 잎을 가해하였지만 볼록총채벌레는 잎과 과실을 심하게 가해하였다. 나방류 해충은 주로 잎을 가해하였지만, 일부 차애모무늬잎말이나방(Adoxophes honmai.) 유충은 망고 과경지를 중심으로 과실표면에 서식하여 과실 피해를 유발하였다. 목화진딧물(Aphis gossypii)과 복숭아혹진딧물(Myzus persicae)은 신초에 피해를 주었고, 가루깍지벌레(Pseudococcus kraunhiae) 등 깍지벌레는 망고 줄기와 과실에 많이 발생하였다. 용과는 개미와 목화진딧물(A. gossypii)에 의한 과실 피해와 담배거세미나방(Spodoptera litura) 유충은 줄기를 심하게 가해하였다. 아떼모야는 주로 나방류 해충인 담배거세미나방과 차잎말이나방(Homona magnanima) 피해가 심하였다.

본 연구에서는 팥의 꼬투리와 줄기를 가해하는 Ostrinia속 해충에 대해 종 동정 과정을 기술하였고, 사육하면서 관찰된 발육특성들을 보고 하였다. 수컷의 생식기는 3-lobed uncus 형태였으며, 가운뎃다리 종아리마디에는 털을 많이 갖는 것으로 나타났다. 미토콘드리아 cytochrome oxidase I (COI)과 II (COII) 유전자의 부분 염기서열은 콩줄기명나방(O. scapulalis), 큰섬들명나방(O. zaguliaevi), 큰조명나방(O. zealis bipatrialis)들에 대해 일본과 중국에서 보고된 서열들과 100% 일치를 보이는 종은 없었다. 기주식물 범위는 국내외 보고들 간에 일치하지 않았다. 암컷 성페로몬샘 추출물의 가스크로마토그래피 분석에서 큰섬들명나방과 큰조명나방의 성페로몬 성분인 (Z)-9-tetradecenyl acetate는 검출되지 않았다. 이상의 결과들을 종합하여 고려하였을 때, 본 연구의 팥 해충을 가해하는 곤충 종은 콩줄기명나방(O. scapulalis)일 것으로 추정되었다. 가을 야외에서 채집된 유충들을 야외조건에서 보관하였을 때, 이듬해 6월과 7월 사이에 성충들이 우화하였는데, 이 결과로부터 본 곤충 종은 말령 유충 단계에서 겨울휴면을 하는 것으로 추정되었다. 이외에 반합성 인공사료를 이용하여 실내 사육이 가능하였다.

In 1990, the human genome project had begun with three billion dollars of budget, and the sequencing and analysis result of the three billion base pairs of human genome was finally published in 2000 to open a new era of genomics. Since the human genome project, many other genomes of eukaryotic model organisms, such as mouse, Drosophila, Arabidopsis, C. elegance, etc., became available, and this led the development of computational biology and comparative genomics. Also, during the last decade, the speed of the nucleotide sequencing increased significantly with lower cost by next generation sequencing technology, and the computational power to handle sequence information also has grown exponentially to make possible that a genomics approach is an affordable tool for many of the biological studies. In the entomology area, the 5000 insect genome project was launched in 2011 for understanding of the biology of insects in a new dimension. Based on the recent studies of functional genomics and the new discoveries in the biological sciences, such as innate immune system, RNAi technique, insect pathogens, etc., the information from the insect genomics study will make possible to improve our capability to manage insect pests in the future.

 ,  , An unknown planthopper was discovered in 2010 in an apple orchard in Sinpoong-myun, Gongju-si and in a blueberry orchard in Deogsan-myun, Yesan-gun. This pest have arrived 4 or 5 years ago and the population density then rapidly increased. It was identified as Ricania sp. by Dr. Murray J. Fletcher. Adult Ricania sp. was found on 26 plants including blueberry, grape, peach, sumac, japanese angelica, jujube, kiwi, Rubus coreanus, apple, apricot, plum and chestnut. Females lay eggs on 1-year-old branches. The mean number of eggs laid on apple and plum were 18.7 and 15.3, respectively. The mean length of egg masses on apple and plum were 12.35 and 11.45 mm, respectively. The distance between eggs laid on apple and plum branchs were 1.3 and 1.5 mm, respectively. The mean number of eggs in the same length of egg mass on apple and plum branchs were 17.1 and 13.3 , respectively.

In order to achieve the optimized pest control, correct estimation of pest densities is a prerequisite to monitor pest damage and to provide efficient pest management plans. Parameters regarding diffusion (e.g., diffusion constant) and population size (e.g., growth rate) were estimated by using diffusion equation. The time series dispersal data of Whiteflies collected in greenhouse were used for modeling. Cross-correlation analysis was conducted to reveal the range and direction of pest population invasion. Sampling theory was further investigated regarding estimation of densities, and population dynamics of Whiteflies were discussed in two dimensions.