Balloon flower (Platycodon grandiflorum A. DC.) is a perennial plant of mainly Campanulaceae family, which have been widely used as a food ingredient and herbal medicine in East Asia. Although demands on related products and yearly cultivation area for balloon flower are increasing, diverse fundamental technologies and molecular breeding studies are not very well supported in Platycodons. In this study, 30 random amplification of polymorphic DNA (RAPD) primers were test in an attempt to explore genetic diversities. In addition, sequences information of the actin gene, a well conserved gene encoding a globular protein that forms microfilaments, was retrieved and analyzed. Two actin homologs were recovered; 3.4 kb fragment is a Pg-actin and 1.4 kb fragment is a Pg-actin homolog with 28.6% similarity. We have confirmed that the Pg-actin gene is configured into 4 exons and 3 introns. A single nucleotide polymorphism (SNP), G↔A, was detected on the intron 3, which served as a target for the CAPS marker development. The marker Pg-Actin-Int3 was applied to 32 balloon flower accessions. Balloon flower DNA sequence information generated in this study is expected to contribute to the analysis and molecular breeding and genetic diversity analysis of balloon flowers.

본 연구의 목적은 하도의 형상이 불규칙한 자연하천에서 2차원 흐름 특성을 해석하고 예측하기 위해 2차 요소를 이용한 정확하고 효과적인 상향가중 유한요소모형의 개발에 있다. 모형의 개발을 위해 선형 삼각형 요소, 선형 사각형 요소와 혼합요소를 적용하였고 2차 삼각형, 사각형 요소와 혼합요소를 적용하여 모형을 개발하였으며, 지배방정식의 수치적분식으로 Gauss Quadrature 방법을 사용하였다. 개발된 모형의 적용성 검증을 위해 하상융기가 있는 수로,