Gonadotropin-releasing hormone (GnRH) is a key neuropeptide regulating reproduction in humans and other vertebrates. Here, we evaluated the reproductive control system mediated by GnRH in the Pacific abalone Haliotis discus hannai. We cloned a prepro-GnRH cDNA (Hdh-GnRH) from the pleural-pedal ganglion (PPG) in H. discus hannai, and analyzed its spatiotemporal gene expression pattern. The open reading frame of Hdh-GnRH encodes a protein of 101 amino acids, consisting of a signal peptide, a GnRH dodecapeptide, a cleavage site, and a GnRH-associated peptide. This structure and sequence are highly similar to GnRH-like peptides reported for mollusks and other invertebrates. Quantitative polymerase chain reaction demonstrated that Hdh-GnRH mRNA was more strongly expressed in the ganglions (PPG and cerebral ganglion [CG]) than in other tissues (gonads, gills, intestine, hemocytes, muscle, and mantle) in both sexes. In females, the expression levels of Hdh-GnRH mRNA in the PPG and branchial ganglion (BG) were significantly higher at the ripe and partial spent stages than at the early and late active stages. In males, Hdh-GnRH mRNA levels in the BG showed a significant increase in the partial spent stage. Unexpectedly, Hdh-GnRH levels in the CG were not significantly different among the examined stages in both sexes. These results suggest that Hdh-GnRH mRNA expression profiles in the BG and possibly the PPG are tightly correlated with abalone reproductive activities.

전복 음료를 개발하기 위하여 전복에 식물류를 혼합한 전복 복합물의 in vitro 항산화능을 측정하였으며, 정상군, 대조군, 전복 복합물의 성인 1일 1회분(APM-1) 급이군(EAPM-1) 및 3회분(APM-2) 급이군(EAPM-2)으로 구분하여 4주간 실험사육하였다. 전복 복합물의in vitro 항산화능과 총 페놀 및 플라보노이드 함량은 APM-2가 유의적으로 높았다. 혈중 총 지질 함량은 대조군에 비해 운동과 전복 복합물을 병행급이한 EAPM-2군이 유의적으로 감소되었다. LDH 활성 및 BUN 함량은 대조군에 비해 전복 복합물 급이군에서 유의적으로 감소되었다. AST 활성은 전복 복합물 급이 시 대조군과 유의차가 없었으며, ALT 및 ALP 활성은 대조군에 비해 유의적으로 감소되었다. 혈청 및 간 조직에서 지질과산화물 함량은 대조군에 비해 전복 복합물 급이군에서 유의적으로 감소하였다. 간 조직의 라디칼 소거능은 대조군에 비해 전복 복합물의 급이 시 유의적으로 증가하였다. 따라서 전복 복합물은 항산화능 및 페놀 화합물에 의존하여 트레드밀 운동에 의한 산화적 스트레스 해소 및 피로 회복 에 도움이 될 것으로 예상된다.

본 연구는 전복내장을 이용하여 젓갈을 제조과정 중 이 화학적 변화와 제조된 젓갈의 품질특성에 대하여 조사하였다. 전복내장의 영양학적 성분을 분석한 결과 탄수화물, 단백질 및 미네랄이 풍부하여 젓갈발효에 우수한 성분특성을 갖고 있었다. 젓갈 발효중 pH는 경시적으로 감소하는 경향을 보였으며 염도가 pH 변화에 영향을 미치지 않았다. 휘발성염기질소함량은 발효중 염농도에 따라 현저한 영향을 받았으며 고염발효의 경우 저염발효에 비하여 휘발성염기질소 함량이 현저하게 낮음을 알 수 있었다. 아미노태질소 함량은 발효중 꾸준히 증가하는 경향을 보였으며, 염농도가 낮을수록 높은 값을 보였으나 염농도에 따른 변화는 크지 않음을 알 수 있었다. 발효과정중 protease 활성 변화를 측정한 결과 고염에서 활성이 떨어지는 경향을 보였으며 발효 30 일경 최고 활성을 보였다. 전복내장과 발효 전복내장젓갈의 유리아미노산 함량을 분석한 결과 젓갈 중 7종의 아미노산이 내장에 비하여 낮게 나타났으며 26 종의 아미노산이 높게 나타났다. 전복내장 젓갈의 총유리아미노산 함량은 62.75 mg/g 으로 전복내장 30.37 mg/g에 비하여 약 2배 증가하였다. 관능평가 결과 색과 종합기호도에서 우수하였으며 상품화를 위해서는 맛과 향의 개선이 필요한 것으로 판단되었다. 전복내장젓갈에 대한 최초의 연구논문으로 향 후 타 연구에 기초자료를 제공할 수 있을 것으로 생각된다.

For the study of population genetic structure with mtDNA, it is essential to measure genetic diversity at each mtDNA regions. Also, to evaluate the variation according to the each region should follow as well as to see if there are differences. In this study, we delved into the variations and dendrogram among samples of seven mtDNA regions (NDⅡ, NDⅤ, NDⅣ, NDⅣL, NDⅥ, NDⅠ, 12SrRNA) from wild Pacific abalone, Haliotis discus hannai collected in Yeosu, Korea. The region with the highest genetic variation was NDⅣ region (Haplotype diversity = 1.0000, Nucleotide diversity = 0.010823) with two to five times higher variation than the others. Furthermore, the study to see if there is a difference between the regions of samples showed that similar aspects of dendrogram in NDⅡ and NDⅠ(divergence of 90% and 87%), which forms a group with hd4, 7, 8 and 10 at bootstrap support, based on 1000 replications. Also, pair-wise FST between clusters within the regions showed high values; 0.4061 (P=0.0000), 0.4805 (P=0.0000) respectively. Therefore we can infer that it is the most efficient and accurate way to analyze the region of NDⅣ with the highest variation in addition to the regions of NDⅡ and NDⅠ, which formed clusters with high bootstrap value, for study of population genetic structure in this species.

In this study, the general compositions, amino acids, fatty acids and collagen content of three abalones, which were harvested in Wando, Heuksando and Jeju Island in Korea, were analyzed and compared. The abalone body and viscera were separated from each other and the physicochemical properties were investigated. The moisture and protein contents of the viscera were lower than that of the body; while the lipid, carbohydrate, and mineral contents were higher. The general compositions and mineral contents also presented fluctuation among the three abalone groups. The amino acid content via hydrolysis in the abalone viscera ranged from 93.16 to 127.02 mg/g, which was lower than the amino acid content of the abalone body of approximately 145 mg/g. However, the free amino acids level in the abalone viscera ranged from 16.81 to 20.10 mg/g, which was higher than that of the abalone body level of approximately 7.90 to 10.59 mg/g. The fatty acid analysis revealed that while the saturated fatty acid percentages in the abalone body were higher than in the abalone viscera, the percentages of the monoenoic and polyenoic fatty acids of the body were significantly lower. The body of the abalone harvested in Wando contained the highest level of collagen (2.26±0.46 mg/g), followed by the abalone harvested in Jeju Island and Heuksando. The abalone viscera contained much lower collagen, which ranged from 0.37±0.15 mg/g to 0.20±0.03 mg/g. The results of this study will provide useful information for the future research of abalone.

In this study, the anti-oxidant and anti-elastase activities of four abalone viscera extracts were investigated to screen the most promising extract. This extract was further studied in terms of its anti-skin-aging properties. In the DPPH-scavenging assay, the Tris-HCl extract showed a 58.60±0.88% radical-scavenging activity, which was followed closely by the ethanol extract that had a 55.40±0.62% scavenging activity. In the anti-elastase assay, however, the ethanol extract showed the significantly highest elastase inhibition activity. Furthermore, none of the extracts had a harmful effect on the human dermal fibroblast, as revealed in the MTT assay. In the cell study, the effect of the ethanol extract at various concentrations on the human dermal fibroblast was investigated. At the 10 μg/mL concentration, the ethanol extract boosted the pro-collagen type I synthesis to 705.30±3.06 ng/mL and reduced the MMP-1 to 54.30±0.80 ng/mL, which was considered the optimum concentration. This is the first study that focused on the anti-oxidant and anti-skin-aging effects of abalone viscera extract. Its results may provide fundamental data for further study.

제주도 성산 연안 지역에 서식하는 오분자기의 생식주기를 조사하기 위하여 2006년 5월부터 2007년 4월까지 채집된 개체를 대상으로 생식소지수와 생식세포 발달단계, 황색 과립세포 출현양상과 생식상피의 형태변화를 조사하였다. 생식소지수(GI)의 월별 변화는 암컷과 수컷 개체 모두 유사한 경향을 보였다. 암컷 GI는 2006년 5월 에서 6~8월 으로 최대값을 보였고, 9월 으로 감소하였다가 10월 증가한 후 2007년 4월까지 낮은 값()을 유지하였다.

결빙억제제(CPA)의 종류, 농도 및 침지시간이 참전복 Haliotis discus hannai 발생배의 생존활성에 미치는 영향을 조사하였다. 4세포, 담륜자 및 피면자를 dimethyl sulfoxide, ethylene glycol, propylene glycol 농도별로 10분, 20분 및 30분간 침지하였다. CPA 침지 후 각 발생배의 발생진행률은 각 CPA 농도가 낮을수록, CPA 침지시간이 짧을수록 높았다. 또한, 피면자에서 비틀림전까지의

본 연구에서는 자연산 말전복과 양식산 참전복의 일반성분을 비롯한 지방산, 아미노산 및 핵산관련물질을 비교 분석하여 전복에 대한 기초자료로 활용하고자 하였다. 자연산 말전복과 양식산 참전복을 육과 내장으로 분리하여 실험한 결과, 육의 경우 자연산 말전복이 양식산 참전복에 비해 수분함량이 높게 나타났으며, 내장도 동일한 결과를 나타냈다. 조단백질 함량은 자연산 말전복에 비해 양식산 참전복에서 더 높게 나타났고, 조지질 함량은 자연산말전복과 양식산 참

전복의 생육 단계, 즉 1, 2, 3 및 4년생의 육과 내장의 이화학적 성분분석 결과를 비교 하였다. 육의 조단백질은 4년생이 로 가장 많은 함량을 보였다. 조지방은 4년생이 로 가장 많았으며, 회분은 1년생이 로 2년생의 와는 유의적인 차이로 가장 많은 함량 이었다. 그리고 3, 4년생 전복의 탄수화물 함량은 1, 2년생과는 유의적인 차이로 다소 높은 함량 이었다. 육과 내장을 비교하면 육이 수분과 조단백질 함량이 많고, 내장이 조지방, 회분

생전복과 3가지 건조방법, 음건법, 냉풍건조법 및 진공동결건조법으로 건조한 전복의 육과 내장에 대하여 이화학적 성분을 분석 비교하였다. 생전복 육은 수분이 , 조단백질 및 탄수화물 이었다. 건조 전복 육의 경우 수분은 냉풍건조가 로 가장 많았으며, 진공동결건조 한 것이 로 가장 적은 함량을 보였다. 생전복 내장은 육과 비교하여 조단백질은 적었지만 다른 성분은 많았다. 생전복은 구성아미노산 총함량이 이고, glutamic acid가 로 가장

This paper describes the amount of food consumption and the change of total weight of abalone under a vibration with noise that can be occurred due to piling work. This experiment was conducted in the aquarium in Yosu National University. In normal situation the juvenile stage shell`s(total length is 1～1.5cm) amount of food consumption was 0.81g, the middle stage shell(total length is 3～3.5cm) was 13.61g, and the adult stage shell (Total length is 7～7.5cm) was 43.19g per 5 organisms in 24 hours, while the experimental group was observed low numerical value compared normal groups. The abalones' food consumption and total weight in both groups, the intermittent and continuance impact with noise and vibration, was reduced during this experiment. The abalones' food consumption and total weight in the experimental groups without vibration were recorded slightly high numerical value than the experimental groups with noise and vibration. Based on this experimental data we could conjecture the noise and vibration are harmful factors to bring up a physiological stress to abalones. Especially, the vibration impact by piling works could produce a considerably unfavorable effect to the abalones than noise impact.

In present study, attempts were made to preserve abalone (Haliotis discus hannai) sperm in liquid form at low temperature, to evaluate the effect of various diluents in short-term storage on sperm, and cryopreservation procedures were optimized for the cryoprotectants as well as freezing rates, in terms of the motility and survival rate, and the ultrastructural changes of sperm after short-term storage and cryopreservation were observed. The abalone sperm reached maximum motility until about 4min after activation. The motility was constant for about 16min, after which it dropped gradually, and about 50min later all motility ceased. Threshold activation of sperm was found in 40% artificial seawater (ASW), and motility increased as the concentration of ASW increased. In Hanks balanced salt solution without calcium (Ca-Free HBSS, 300 and 400 mOsmol/kg) and 10%, 20%, and 30% ASW the sperm was immotile, and motility once again restored incompletely only in HBSS of 300 and 400 mOsmol/kg, 20% and 30% ASW after 100% ASW was added. Sperm motility was extended following 20 days of cold storage only in 70% and 100% ASW. A high motility index of 3.5-4.5 was observed for the first 8 days in 70% and 80% ASW. In other diluents sperm motility was constant less than 10 days, and the motility index was obviously lower than that of sperm in 70% and 100% ASW. After 20 days of cold storage survival rates of 10.2%-20.7% were obtained in ASW and 300 mOsmol/kg HBSS, and that in 400 HBSS (65.3%) was significantly higher than others. The constant period of sperm motility stored in 70% ASW was longer obviously than that in 100% ASW after 6 days of storage, and the time to maximum motility of sperm stored in 70% increased gradually, while the difference in which of sperm in 100% ASW was not significant. The sperm plunged into liquid nitrogen all died except that sperm using 15% glycerol as cryoprotectant restored 10.4% of motility. The highest motility index (3.4) was obtained with 5% glycerol and freezing procedure: 50℃/min from to .