Biodiesel is a remarkable alternative fuel for the diesel engine among various countries. Various studies are carried out on the environmental and economic effects as well as the nature of diesel engine fuel biodiesel in the combustion process and emission characteristics. In this study, CRDI diesel engines used diesel fuel and 20 % biodiesel blend fuel (BD 20 %) as fuel, with City-mode exceeding 300 hours. Engine performance and emissions were sampled at one-hour intervals for analysis. The engine was inspected after testing to check the engine parts (valves and injectors). It was concluded that 300 hours of operation using BD 20 % does not cause abnormal deterioration of the engine or abnormal changes in engine power and exhaust gas.