Purpose: The purpose of this study was to understand the nurse’s experiences of ‘work flow interruption’ in hospital settings. Methods: Data were collected by in-depth interviews with 6 nurse in General wards or in the ER of tertiary hospitals. Qualitative content analysis method was used to analyze the data. Results: The following three categories were elicited; ‘acceptable interruptions’, ‘unacceptable interruption’, ‘nursing work environment interrupting work flow’. Conclusion: The findings provided interruptions are occurring in systemic structure and relationships within the hospital, taking into account the aspect of providing quality nursing care as profession. This confirms that nurses have negative effect on workload rather than positive ones. Based on the findings, it needs to be of interest to the nurses at the organizational level in order to relieve the burden and fatigue due to overloaded work.