The experiment was conducted to determine effect of the mixed herbal medicine for the substitution of antibiotics on the performance of laying hens. Day old hyline 1,500 layer chicks were randomly assigned to 4 treatments. Control were 600 chicks and there were three treatments of each 300 chicks. The 4 treatments were as follows: the mixed herbal medicine 0.1%(T-1), 0.3%(T-2) and 0.5%(T-3) after removed antibiotics on commercial feed and commercial feed(control) from 5th week to 13th week, each treatment was replicated 3 times and from 14th week to 18th week. Each 100 hens of the mixed herbal medicine 0.1%(CT-1), 0.3%(CT-2) and 0.5%(CT-3) were moved to cage. Body weight were measured on 4th, 8th, 13th week and feed intake, mortality were measured on every weeks. Body weight at fourth week, all treatments tended to be higher than control and T-3 statistically was highest(p<0.01). On 8th week, also treatments statistically high and T-1 was highest(p<0.01). But 12th week, there was not significantly different among treatments. Therefore it will be possible that the mixed herbal medicine substitute for antibiotics after vaccination. Mortality was not different between treatment and control overall rearing period. Early laying period(19～41wk), T-2 showed highest feed intake(107.1g) among treatments, later laying period(42～77wk), T-1 showed highest feed intake(134.3g) and was not any different among each treatments.