Effective stress management in pilots is crucial for flight safety. This study assesses the stress levels of student pilots enrolled in a private pilot course during their initial flights by measuring heart rate variability (HRV) using a wrist watch type heart rate monitoring device. HRV data were collected from 31 student pilots across their first ten flight sessions. Statistical analysis using repeated measures ANOVA revealed that the highest stress levels occurred during the first flight and gradually decreased over subsequent flights. These findings highlight the need for flight instructors to provide supportive guidance during initial flights to help students adapt to flight conditions with minimized stress. Furthermore, the private pilot course curriculum may require revision to incorporate stress management and flight aptitude evaluation. This research contributes important insights into monitoring physiological stress responses in trainee pilots and informs strategies to enhance pilot training effectiveness and safety.