In this paper, cycle performance analysis of two-stage compression and one-stage expansion refrigeration system using alternative Freon refrigerants is presented to offer the basic design data for the operating parameters of the system. Pure alternative Freon refrigerant R32, R134a and near NARM(Non-Azeotropic Refrigerants Mixtures) R404A, R407C, R410A and ARM(Azeotropic Refrigerant Mixtures) R507A were used working fluids in this study. The operating parameters considered in this study included evaporation temperature, condensation temperature, subcooling degree, superheating degree, mass flow rate ratio of inter-cooler. The main results were summarized as follows : The COP of two-stage compression and one-stage expansion refrigeration system increases with the increasing subcooling degree and mass flow rate ration of inter-cooler, but decreases with the increasing evaporating temperature, condensing temperature and superheating degree. Therefore, subcooling degree, mass flow rate ratio of inter- cooler of two-stage compression and one-stage expansion refrigeration system using alternative Freon refrigerants have an effect on COP of this system.