The objective of this study is to investigate the earthquake response for the design of 100m spanned single-layer lattice dome. The plastic hinge analysis and eigenvalue buckling analysis are performed to estimate the ultimate load of single-layered lattice domes under vertical loads. In order to ensure the stability of lattice domes, it is investigated for the plastic hinge progressive status by the pushover increment analysis considering the elasto-plastic connection. One of the most effective methods to reduce the earthquake response of large span domes is to install the LRB isolation system of a dome. The authors discuss the reducing effect for the earthquake dynamic response of 100m spanned single-layered lattice domes. The LRB seismic isolation system can greatly reduce the dynamic response of lattice domes for the horizontal and vertical earthquake ground motion.