This study aims to estimate the species, size and shape of fish using a non-contact 3 dimensional pattern laser so that this preliminary test was carried out to understand the structural feature and length of goldfish according to water turbidity and depth in the aquacultural tank. 3-D pattern laser could clearly detect its morphological shape except the caudal fin due to soft tissue. Since the sensing strength of line laser light according to depth has sufficient power, it is possible to measure its depth and structural feature in the detected range. The result showed that the measured error of individual’s fork length was less than ±1% in the water using 3-D pattern laser, when compared with the measured value in the air.