The purpose of this study was to develop an in-service education program on multifunctionality of agriculture and rural areas. The in-service education program for general agricultural education was developed by reviewing literature, analyzing domestic in-service education programs. The program consisted of 3 fields - understanding, experiencing multifunctionality, and applying it to classroom, and contents validity was evaluated by 3 experts. And the program was applied to 268 elementary and secondary school teachers. Before and after the in-service education program, the survey was conducted. The survey questions were about motivation of participation, satisfaction level of program, and so on. Based on the analysis of survey data, some recommendations were suggested.