The separation method of Au (III) was studied by a chelating resin, Amberlite A-21. Sorption capacities for the metal ions on the Amberlite A-21 have been measured by batch method. This resin was applied to the rapid concentration of trace amounts of these metal ions and to the separation of Gold (III) from other metal ions in bulk solution. The separation of those elements was carried out with Acetone and Thiourea as eluents.