본 연구는 소루쟁이 뿌리 추출물의 유효성 실험을 위해 항산화 활성 및 항균 효능을 확인하고 제형 안정성을 확인하였다. 항산화 활성으로는 DPPH radical scavenging, FRAP activity, ABTS+ radical scavenging, SOD-like activity를 진행하였으며 항균 활성 평가는 Staphylococcus epidermidis, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Candida albicans 균주에 대해 생육저 해환과 최소저해농도를 평가하였다. 또한 소루쟁이 뿌리 추출물을 함유한 스킨을 21일 동안 pH, 온도, 일 광에 대한 경시 변화를 확인하였다. 항산화 평가 결과 0.0625-1mg/mL 농도에서 농도 의존적으로 활성이 증가하는 것을 확인할 수 있었다. 생육저해환의 경우 100mg/mL 농도에서 각 균의 생육저해환이 10.45±0.34, 9.77±0.59, 9.92±0.22, 10.08±0.12로 대조군인 Methyl paraben에 비해 우수한 항균력을 확인할 수 있었고 최소저해농도의 경우 100mg/mL 농도에서 S. aureus, E. coli에 대한 항균력을 확인하였 다. 스킨의 pH 농도가 4.0, 6.0, 7.0에서 흡광도의 변화가 미미하였고, 4℃, 25℃, 40℃에서 온도가 높아질 수록 변색되는 것을 확인하였다. 또한 스킨을 일광과 실온에서 보관했을 때 일광에서 변색이 일어난 것을 보아 소루쟁이 뿌리 추출물을 함유한 화장품은 차광하여 저온 보관하면 변색을 방지 할 수 있을 것으로 추 측된다. 본 연구 결과를 종합하였을 때 소루쟁이 뿌리 추출물은 항산화, 항균 활성을 기대할 수 있는 화장 품 원료로 이용 가치가 높을 것으로 사료된다.

Salmonellosis is a widespread bacterial zoonosis that commonly causes enterocolitis and foodborne poisoning leading to an extensive economic loss in domestic animal industry. Considerably, the emergence of multidrug resistant strains of Salmonella spp. induces further severe problems affecting public health. The present report was designated to investigate the antibacterial efficacies of three common disinfectants including an oxidizing compound disinfectant (OXC), a triple salt (TS) and a quaternary ammonium compound (QAC) against Salmonella typhimurium subjected to the preliminary changes of drug temperature. All solutions of three disinfectants were pre-incubated at different temperature (22, 37 and 63°C) for 1 h prior to exposure to bacteria. The disinfectants and bacteria were diluted with distilled water (DW), hard water (HW) or organic matter suspension (OMS) according to treatment condition. Under the DW condition, the disinfectant efficacy of the QAC at 63°C was higher than that of 22°C. Furthermore, under HW diluent the disinfectant efficacy of the TS pre-warmed at both of 37 and 63°C were increased compared to that of 22°C. Considerably, the efficacy of pre-warmed QAC at both of 37 and 63°C under the OMS diluent were higher than that of 22°C. Conclusively, prewarming at higher temperatures have positive effects on the stability of the antibacterial efficacies of TS and QAC.

The entomopathogenic fungi were an important natural pathogenic of insects that has been developed as potential biological control agents for many important agricultural, forest and medical pests. Several these fungi produce a wide range of secondary metabolites with high therapeutic value as antibiotics, cytotoxic substances, insecticides, compounds that promote or inhibit growth, attractor and repellent. Therefore, this study was performed to select the antibacterial activity of liquid culture filtrates of 347 entomopathogenic fungi form Korea soils against two pathogenic bacteria including Ralstonia solanacearum and Escherichia coli using novel method which represents a quick and easily applicable tool obtaining large number of samples. As results, eight-five strains (24%) and seventy-six strains (22%) of these fungal metabolites produced anti-R. solanacearum and anti-E. coli compounds, respectively. The preferential antibacterial activity against R. solanacearum and E. coli gives evidence that these entomopathogenic fungal metabolites might be useful as an agent for bacteria control and the technique was simple to operate and allowed a large number of samples to be handled concurrently.