The exotic aphid pest, pomegranate aphid, Aphis punicae Parsserini, 1863 (Hemiptera: Aphididae), is newly recognized in the Korean peninsula. This species is originally distributed throughout the Mediterranean regions, the Middle East, Ethiopia, India, Pakistan, Indonesia, and Turkey. In the major producers of pomegranate such as India and the Mediterranean regions, this species is regarded as one of the most serious insect pests inflicting injury on pomegranate, Punica granatum Linnaeus. By sucking plant sap and excreting honeydew, aphids cause various plant diseases and virus infections which are more likely to occur as population size increases. Aphis punicae infests together commonly with Aphis gosspii Glover, and rarely with Aphis craccivora Koch and Aphis spiraecola Patch on P. granatum in Korea. In this study, we first compared two closely related species, A. gossypii and A. punicae, based on morphometric analysis to confirm their morphological relationships. Second molecular identification using mitochondrial COI barcode region was performed for the aphid samples collected from P. granatum.