In this study, we discussed about the application of the single physical and chemical treatment processes and the physical-chemical complex treatment processes on the inactivation of Artemia sp. in order to satisfy the USCG Phase Ⅱ (United States Coast Guard). The results showed that initial disinfection rate of ultrasonic process in single batch process is higher than that of electrolysis. However, the inactivation rate showed slower than electrolysis. The inactivation rate of Artemia sp. on the single continuous treatment process ranked in the following order: homogenizer 〉 electrolysis 〉 ultrasonic process. Inactivation rate of Artemia sp. in continuous homogenizer-electrolysis complex process was reached at 100% immediately. A synergistic effect of ultrasonic–electrolytic complex process was found to be a small. The order of processes in a complex process did not affect the disinfection performance.