In this study, the damping ratio was estimated using the developed automatic calculation software based on the field measurement data of vibration of stay cable in cable-stayed bridge. For the field measurement, two accelerometers were used for obtaining of dynamic behavior of 1st, 2nd and 3rd mode. The damping ratio after installation of the damper was calculated as higher than the requirement values, also it was confirmed that the damping effect was improved with the range of 19~92%.