Lysiphlebus orientalis is previously recorded in 2010, which is a more recently recorded than Lysiphlebia japonica. Host range of Lysiphlebus orientalis range is narrow, while that of Lysiphlebia japonica is very broad. Although two species, Lysiphlebus orientalis and Lysiphlebia japonica, are belonged to different genus, respectively, they are morphologically similar each other, which make us confused. Therefore, we have to identify these two cryptic species using COI DNA barcode. We used the ‘NCBI-BLAST (National center for biotechnology information-Basic Local Alignment Search Tool)’ to perform COI DNA barcode identification, and introduce preliminary results in this presentation.