Widespread use of chemical insecticides has resulted in development of insect resistance and natural products withbiological activities could become an attractive alternative to control insect pests. In order to find more effective insecticidesfor controlling mosquito, various mosquitocidal compounds are studied. Recently, juvenile hormone antagonists (JHANs)are known to be to safe and effective insecticides for control mosquito. In order to identify novel insecticidal compoundswith JHAN activity, several chalcones were surveyed on their JHAN activities and larvicidal activities against Aedes albopictuslarvae. Among them, 2’-hydroxychalcone and cardamonin showed high level of JHAN and mosquito larvicidal activities.These results suggested that chalcones with JHAN activity could be useful for control of mosquito larvae.