This study was conducted to determine optimum design parameters in nitrification and denitrfication of chemical fertilizer wastewater using pilot plant, Jet Loop Reactor. The chemical fertilizer wastewater which contains low amounts of organic carbon and has a high nitrogen concentration requires a post-denitrfication system. Organic nitrogen is hydrolyzed above 86%, and the concentration of organic nitrogen was influent wastewater 126㎎/L and of effluent wastewater 16.4㎎/L, respectively. The nitrification above 90% was acquired to TKN volumetric loading below 0.5 ㎏TKN/㎥‧d, TKN sludge loading below 0.1 ㎏TKN/㎏VSS‧d and SRT over 8days. The nitrification efficiency was 90% or more and the maximum specific nitrification rate was 184.8 ㎎TKN/L‧hr. The denitrification rate was above 95% and the concentration of NO3-N was below 20㎎/L. This case was required to 3 ㎏CH3OH/㎏NO3-N, and the effluent concentration of NO3--N was below 20㎎/L at NO3--N volumetric loading below 0.7 ㎏NO3--N/㎥‧d and NO3--N sludge loading below 0.12 ㎏NO3--N/㎏VSS‧d. At this case, the maximum sludge production was 0.83 ㎏TS/㎏T-Nre and the specific denitrfication rate was 5.5 ㎎NO3-N/ gVSS‧h.