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        검색결과 2

        1.
        2018.03 KCI 등재후보 구독 인증기관 무료, 개인회원 유료
        Cluster of differentiation (CD) 24 or heat stable antigen 24 (HSA) molecule is a mucin-type glycoprotein attached to the cell surface by glycosylphosphatidylinositol (GPI)-anchor, promoting adhesive interactions between cells or in extracellular matrix. The aim of this study was to determine the not yet fully identified porcine CD24 gene and protein structure using computational analysis and to validate variants reported in exons of CD24 gene using direct sequencing. A total of 59 samples belonging to Yorkshire, Landrace, Berkshire, Jeju black pig and wild boar were used in the study. Human CD24 mRNA sequences were used as a reference and subjected to BLAST searches to retrieve the orthologous expressed sequence tags (ESTs) or cDNA sequences against NCBI and Ensemble databases. Assembled ESTs and retrieved cDNA sequences for the porcine CD24 gene were used for specific BLAST search to determine its genomic structure. We found porcine CD24 gene to consist of two exons and a relatively long intron. Second exon of porcine CD24 gene had a long 3’ untranslated region (UTR) and was very similar to that of human, mouse, rat, and sheep. The sequence homology of porcine CD24 protein was 65.38-84.62%, when analyzed with amino acid sequences of rat, mouse, human, cattle, and sheep CD24 protein. N-terminal signal sequence, O-glycosylation sites and GPI-anchoring signal sites were also predicted in pig, which showed these motifs to be evolutionary conserved across the species. Variant analysis in exonic regions of porcine CD24 among the multiple breeds showed that only second exon contained eight SNPs and three insertions in a 3’ UTR. Taken together, this study reports putative porcine CD24 gene and its protein structure using in silico approaches, which will be helpful for any further functional studies.
        4,000원
        2.
        2002.04 KCI 등재 서비스 종료(열람 제한)
        삽교호유역에 위치한 19개 수질관측지점에서 측정된 월별수질자료를 이용하여 수질관측지점을 2개에서 7개의 수질특성으로 분류하였으며, 그에 따른 수질요인분석을 실시하였다. 군집분석결과 삽교호유역의 각 하천은 개개의 수질특성을 보이고 있으며, 삽교호, 삽효천, 무한천 및 곡교천의 4개 그룹으로 나눌 수가 있었다. 수질분석결과에 의하면 삽교호에서는 부유물질의 농도가 다른 하천보다 높았는데 이는 하천으로부터 유입되는 풍부한 영양염에 의한 식물플랑크톤의 생물량 증