Until recently, molecular detection method for Bursaphelenchus xylophilus (Bx) infesting pine trees and killing themhas been developed several ways such as RFLP, Real-time PCR and LAMP. However, these were not only time-consumingprocess but also could not confirm the result in a short time. Furthermore, these methods also need thermo cycler orthermostat which are not portable and inexpensive. For above reasons, we had been developed the detection method forBx remaining in the pinewood chips without using electronic devices. In this study, we had used DUNT buffer that isable to extract genome DNA from Bx in the pinewood within 10 min and isothermal amplification that could be amplifiedthe specific DNA fragment of Bx at 37 ℃ for 10 min. As a result, our method is able to confirm the presence or absenceof Bx in pine trees within 20 min and could be used in the field as it does not require the electronic devices.