Artificial intelligence (AI) is an interdisciplinary subject based on computer science and has a history of more than 70 years. With the development of social industries, various service needs are developing rapidly, and AI is also playing a huge role in the application field of big data. This paper focuses on the analysis and application of AI in the subject classification system for high school students. The subject classification system adopts the characterization of subjects that a country have; and the amount of information data is huge. Different countries set up different subject systems according to the needs of social structure. In 2013, Qingdao Warwick International Education College (Warwick College) and Korean wisemento Co., Ltd. (Wisemento company) spent 4 years jointly researching and completing an analysis and assessment system suitable for Chinese subject classification system. Follow the subject and professional scoring method, the assessment system scientifically calculates trend scores and interacts with potential and career models to find the subject development direction, professional trend, future potential development direction, subject and career direction that are suitable for students. It provides trend data suitable for students’ subject development, provides scientific basis for students’ course selection, helps students understand themselves, and provides students with targeted career education development directions for subjects and careers. The education resources can be utilized effectively; student individual data also provides a basis for the management of education and teaching.