Through Estimation of stress distribution, both value and location of the maximum stress can be found. and the maximum stress is very important indicator for structural safety evaluation. In this connection, a lot of studies to estimate stress distributions are conducted. But studies on the optimal number and location of measurement points are few. So, In this study, the effects of the number and location of measurement points on the estimation stress distribution for the steel beam structure using motion capture system and cubic smoothing spline interpolation are researched.