The aim of the present study was to evaluate the beneficial effects of Eriobotryae Folium on diabetic improvement through oxidative stress and inflammation in the liver of type 2 diabetic db/db mice. Eriobotryae Folium extract (100 or 200 mg/kg body weight, p.o) was administrated everyday for 3 weeks. And then, its effect was assessed on comparison with vehicle-treated db/db and non-diabetic m/m mice. Serum glucose and hepatic tissue biochemical factors and protein expression related with oxidative stress and inflammation such as reactive oxygen species (ROS) and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase were measured. As a result, the administration of Eriobotryae Folium decreased body and liver weight, food intake in vehicle-treated db/db. Also, serum glucose was lowered by Eriobotryae Folium treatment. Eriobotryae Folium administration decreased oxidative stress through the down-regulation of ROS and NADPH oxidase subunit proteins, NOX-4 and p47 phox .Moreover,theincreaseofinflammatoryproteinssuchasinduciblenitricoxidesynthase(iNO S),cyclooxygenase-2(COX-2),tumornecrosisfactoralpha(TNF-α), interleukin-6 (IL-6) on vehicle-treated db/db were significantly decreased through down-regulation nuclear factor-kappa B (NF-κB) and activator pretoein-1 (AP-1) via reduction of oxidative stress. Therefore, hepatic functional parameters such as alanine aminotransferase, aspartate aminotransferase significantly decreased. In conclusion, Eriobotryae Folium extract could have hepato-protective effect through down-regulation of abnormal NADPH oxidase subunit and the oxidative stress in type 2 diabetic db/db mice.

Prostaglandin (PG) E2 is an important mediator of skin wound healing without excessive scarring and gastric ulcer healing. However, PGE2 has a short lifetime in vivo because it is metabolized rapidly by 15-hydroxyprostaglandin dehydrogenase (15-PGDH). Ethanol extract of Eriobotryae folium (EFEE) elevated intracellular and extracellular PGE2 levels in HaCaT cells and inhibited 15-PGDH (ED50 : 168.4μg/mL) with relatively low cytotoxicity (IC50 : 250.0μg/mL). Real-time PCR analysis showed that mRNA expression of cyclooxygenase (COX)-1 and COX-2 enzymes were increased and prostaglandin transporter (PGT) was decreased in HaCaT cells by EFEE. Moreover, wound healing effect of EFEE (168.4μg/mL) was comparable to that of TGF-β1 (300 pg/mL) as a positive control. These results demonstrate that EFEE may be valuable therapeutic materials for the treatment of PGE2 level dependent diseases.