Erysipelothrix rhusiopathiae is a causative agent of erysipelas in avian and mammalian species globally. Eight dead 5-week-old Ross broiler chickens were submitted for diagnostic tests in September 2005. Typical signs including white necrotic foci on the liver, focal congestion of the pancreas, severe atrophy and hardening of the bursa of Fabricius were detected. E. rhusiopathiae was isolated from haemorrhagic lung samples. However, no specific PCR product for the viral diseases tested was detected in these chicken samples. To our best knowledge, this is the first report of the isolation of E. rhusiopathiae in broiler chickens in Korea.