The purpose of this study was to examine the relationship of fatigue and stress in seafarers. Ninety healthy adults were recruited and cumulative fatigue, stress index and stress resistance of seafarers were measured using autonomic nervous system analysis device (SA-6000; Medicoa Co. Ltd. Korea). The stress index had a significant correlation with the degree of resistance (p<.01). Cumulative fatigue and the stress index had a strong positive correlation (r=.781) with the stress resistance, cumulative fatigue had a strong negative correlation (r=.782) (p<0.01). Furthermore, the stress index had a strong negative correlation with the degree of resistance (r=.924) (p<.01). The present study indicate that there are significant correlation between the cumulative fatigue and stress index, cumulative fatigue and stress resistance, as well as stress index and stress resistance in the seafarers (p<.01).