Demineralized Freeze Dried Bone(DFDB) graft material have been used for reconstruction of large bony defects or augmentation of thin alveolar ridge during implantation of titanium fixtures. But at present osteogenic effect of DFDB do not overcome the capacity of autogenic bone graft. Thus many investigators had applicated various bioactive substance to DFDB to enhance the ability of osteogenesis of DFDB. In this study, mixture of grafting material was made from fibrin glue(F) and DFDB(D)(group 1: F+D), fibrin glue, DFDB and rhBMP-2(B) (group 2: F+D+B), fibrin glue, DFDB, polylactic- co-glycolic acid(PLGA)(P) and rhBMP-2(goup 3: F+D+B+P), fibrin glue, DFDB, PLGA, rhBMP-2 and autogenic osteoblasts( O)(group 4: F+D+B+P+O), and fibrin glue, DFDB, autogenic osteoblasts (group 5: F+D+O). During first surgical procedure, extraction of molar teeth was performed at male Biggle dog's mandible, and collected bone marrow tissue from tibia at same Biggle dog. Collected bone marrow tissue was cultured and differentiated into osteoblasts in vitro, and stored in nitrogen bottle. After four months, titanium fixture was implanted with prepared graft material to Biggle dog's mandible. After four and eight weeks respectively, experimental dog was sacrificed. Obtained tissues were prepared for examination by using resin embedded ground section method. Prepared sections were evaluated with transmitted and polarized microscope, and areas of osteoid and cacified bone were calculated with IPTK 5.0( image processing tool kit version 5.0). Resultant data was statistically analyzed by SPSS 13.0 software. Results of this study showed that autogenic osteoblats had more enhancing capacity of bone formation than rhBMP-2, but PLGA inhibited bone forming potential of bony tissue.