Background: Although several studies have reported functional improvements after forward walking training on a treadmill and after backward walking training on a treadmill, there is a lack of immediate effects data, investigating spatiotemporal parameter, neuromuscular recruitment. Objectives: To compare the immediate effects between forward and backward walking on treadmill training, present study measured muscle activities of the lower extremity, gait parameters, and dynamic balance. Design: Cross-sectional study. Methods: The twenty-four asymptomatic young male subjects were participated in this study. Subjects have performed 15-min of forward and backward walking on treadmill. Before and after treadmill walking, the gait parameters were measured with the GAITRite. The dynamic balance abilities were assessed with the Y-balance test. Muscular activities were collected from the tibialis anterior, gastrocnemius, biceps femoris, and rectus femoris during forward and backward walking. Results: Muscular activities of the biceps femoris and tibialis anterior were significantly different between forward and backward walking conditions, which were higher with the backward walking (P<.05). Compared to the pre-measurement, the normalized dynamic balance composite score of the post-measurement was significantly higher with the backward walking (P<.05). Regardless of the treadmill training method, there were no significant differences between pre and post measurement, both the stride length and step length (P>.05). Conclusion: The performing backward walking training has positive effects for dynamic balance.