This study is a descriptive survey research conducted to confirm the effects of work values and career adaptability on job preparation behavior in Graduation year nursing students. The subjects are 4th grade students who are attending two university nursing departments in city G and N, and data were collected from September to October 2023. The data were analyzed by descriptive statistics, t-test, ANOVA, Pearson’s correlation analysis and controlled regression analysis. The research results are as follows. Intrinsic values (β=-.127, p=.122) had not statistically a significant effect on job preparation behavior. Extrinsic values (β=.168, p<.001) and career adaptability (β=.415, p<.001) had statistically a significant positive effect on job preparation behavior. The variables predicting the job preparation behavior of nursing students were identified in the order of career adaptability and extrinsic values. The study model accounted for 35.5% of job preparation behavior. It suggests that strategies are needed to enhance the extrinsic values and career adaptability in order to promote job preparation behavior in nursing students. However, considering the nature of nursing and the sense of calling of nurses who perform nursing, we should not overlook the fact that the intrinsic values of nursing itself is also an important aspect that nursing students preparing to become future nurses must cultivate.