RDA(Rural Development Administration of Agriculture) and YIRI(Yecheon-gun Industrial Insect Research Institute) was development of 3 strains crossbred honey bee(Apis mellifera) for increasing honey production(HP). The overall goal of this research is to improve the honey production of queen honey bees. This will enhance the economic value of the nation’s honey bees for honey production, and hazard resistance. Our main objective of this research is to test of honey bees(A. mellifera) that have increased as well as being good honey producers and resistance of disease in jeon-nam province. The new honey bee(A. mellifera) stock were identified ability of increasing honey production by comparing with rearing practice colony. The new honey bee(A. mellifera) stock can produce more than 30~50% honey(HP; 12.31 kg) comparing with rearing practice colonies(control 1; 8.17 kg, and control 2; 9.53 kg). Furthermore, we are calculated the number of worker bee per colony. Population of worker bee in new honey bee(A. mellifera) stock are 2,849 (colony 1), 8,860 (colony 2) and 10,451 (colony 3), it was more then 1.2~3.7 fold comparing with controls.