According to ISO 4037, the thickness of the inherent filtration for the radiation qualities L-40 to L- 240, N-40 to N-400, W-60 to W-300 and H-80 to H-400 shall be equivalent to 4 mm Al for matched reference radiation fields or adjusted as far as possible to 4 mm Al for characterized reference radiation fields. And for matched reference fields, the tube window must be made of beryllium and its thickness should not exceed 10 mm. In the case of characterized reference fields, the thickness of the beryllium window should not exceed 10 mm, but it is acceptable to use an aluminum window with a maximum thickness of 1.5 mm. 320 KV X-ray tube installed at KHNP-CRI has been designed to equipped with a 3 mm Be for tube window and an additional 4 mm Al to obtain a total inherent filtration equivalent to that of 4 mm Al. In the previous study, the inherent filtration of 320 kV X-ray tube at KHNP-CRI has been verified by MCNP simulation. However, the ISO standards suggest a method for determining the thickness of the inherent filtration by half-value layer (HVL) measurement and spectrometry. In this regard, the inherent filtration was reassessed using HVL measurement. To determine the inherent filtration, 1st HVL of the beam generated by the tube at a tube potential 60 kV was measured. The measurements were conducted with a calibrated spherical ionization chamber (model A3, Exradine) placed at a distance of 1 m from the target, at the center of the radiation field size. The X-ray tube current was set to 2 mA. The thickness of aluminum absorbers was gradually adjusted in subsequent measurements until approached the 1st HVL. 1st HVL were estimated using the linear regression equation computed with the current values for the thickness of the absorbers. As a results, the thickness of the 1st HVL was estimated as 2.845 mm Al. According to the correlation between the inherent filtration and 1st HVL suggested in ISO standard, the value of the inherent filtration was deduced as 4.25 mm Al that is rounded to the nearest 0.05 mm by interpolation. Further studies on the effects of the inherent filtration thickness determined in this study will be conducted.