Insecticide resistance development of insect pests is one of the main threatening factors against stable crop production and keeping human life safe from insect pest disease vector. To know properties of insecticides resistance is very important to develop a management program against insecticide resistance pests. Insecticide resistance development is an event of evolutionary process. We have to deal with insecticide resistance of insect pests by interaction between insecticide and insect pests in their chemical, physical and biological environment. We can glimpse at and infer the evolutionary process of insects from investigating and comparing the resistance level of insect pests to insecticides. Huge amount and many kinds of insecticides have been sprayed to control insect pests. Development of insecticide resistance of insect such as housefly and mosquito, known as most common medical insect, is a result of adaptation to environment covered with insecticides. We can easily assume that the amount and kind of insecticides sprayed the evolutionary force to the diversity of insecticide resistance. The resistance diversity will be very good character of good markers for determining geographical isolation.