A eukaryotic microalga was isolated from seawater in Janghang Harbor, Korea and its morphological, molecular, and physiological characteristics were investigated. Due to its simple morphology, no distinctive characters were found by morphological observation, such as light microscope or scanning/transmission electron microscopy (S/ TEM). However, molecular phylogenetic evidence inferred from the concatenated small subunit (SSU) 18S rRNA and internal transcribed spacer (ITS) sequence data indicated that the isolate belonged to the newly described Micractinium singularis. Furthermore, it was clustered with Antarctic Micractinium strains and it also showed a psychrotolerant property, surviving at temperatures as low as 5°C. However, its optimal growth temperatures range from 15°C to 25°C, indicating that this microalga is a mesophile. Additionally, gas chromatography-mass spectrometry (GC/MS) analysis showed that the isolate was rich in nutritionally important omega-3 polyunsaturated fatty acid, and high-performance liquid chromatography analysis (HPLC) revealed that the high-value antioxidant lutein was biosynthesized as an accessory pigment by this microalga, with glucose as the major monosaccharide. Therefore, in this study, a Korean marine M. singularis species was discovered, characterized, and described. It was subsequently added to the national culture collections.
Gunsan-Janghang Harbor is located at the mouth of Gum River, on the central west coast of Korea The harbor and coastal boundaries are protected from the effects of the open ocean by natural coastal islands and shoals due to depositions from the river, and two breakwaters. The navigation channel commences at the gap formed by the outer breakwater and extends through a bay via a long channel formed by an isolated jetty. For better understanding and analysis of wave transformation process where a wide coastline changes appear due to on-going reclamation works, we applied the spectral wave model including wind effect to the related site, together with the energy balance models. This paper summarizes comparisons of coastal responses predicted by several numerical wave predictions obtained at the coastal waters near Gunsan-Janghang Harbor. Field and numerical model investigations were initially conducted for the original navigation channel management project. We hope to contribute from this study that coastal engineers are able to use safety the numerical models in the area of port and navigational channel design.