Due to the similarities of their anatomical morphology and physiology to those of humans, pigs have increasingly become useful models for toxicological and pharmacological studies. This study was conducted in order to evaluate the quality control level through hematological features of minipigs. Thus, we attempted to determine whether there are differences in hematological parameters between male and female minipigs. No significant differences in the levels of erythrocytes, hematocrit, mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), leukocytes (WBC), and serum electrolytes were observed between male and female minipigs. However, leukocytes were higher than reference range in both sexes (26.46±2.06 vs 11.6±5.4 for male; 23.58±3.36 vs 10.0±2.0 for female). In differential leukocyte counts, no significant differences in leukocyte parameters were observed between sexes. However, neutrophils were higher than reference range in both sexes (60.65±13.92 vs 33.1±18.9 for male; 46.84±12.80 vs 15.4±5.6 for female). In serum biochemical analysis, no significant differences in biochemical parameters were observed between male and female minipigs. However, aspartate aminotransferase (AST) and alkaline phosphatase (ALT) were higher than reference range, while alanine aminotransferase (ALP) was lower than reference range. In urine analysis, pH of male and female was 8.3±1.0 and 6.4±1.6, showing a higher value in male minipigs. These data indicate that quality control of minipigs must take precedence in conduct of toxicological and pharmacological studies.