본 연구에서는 Ocean Data View (ODV)를 이용하여 한반도 주변 해역의 수온·염분 분포도를 작성하는 실험 프로그램을 개발하였다. 정선 해양 관측 자료는 본 연구를 위해 새롭게 개발된 별도의 프로그램을 이용하여 ODV 호환 형식으로 변환된 후 분석되었다. 새로운 실험 프로그램을 65명의 예비 교사들에게 적용하였을 때, 기존 프로그램을 사용한 같은 집단에 비해 과제 수행 완성도가 매우 향상되었다. 새로운 프로그램에 대한 실험 참가자들의 만족도를 살펴보기 위해 설문지 분석을 실시하였다. 분석 결과 정확하고 빠른 등치선 작성 분야에서 가장 높은 만족도를 나타내었으며, 새로운 실험 프로그램의 이해 및 적용에 관한 긍정적인 반응을 확인하였다.

The mechanisms by which embryo exactly distributes mitochondria into the blastomeres during embryogenesis are one of the important issues in developmental biology. Although the mechanisms has been thought to be important for the proper embryonic development, our understanding has remained limited. In the present study, the distribution of mitochondria was examined in embryos of the ascidian, Halocynthia roretzi, by immunohistochemical staining with three-types of the mitochondria-specific antibodies and vital staining of mitochondria with a fluorescent probe, DiOC2(3). Results of the immunohistochemical staining coincided with that of vital staining, which is able to detect the distribution of mitochondria in cytoplasm of the embryo. Mitochondria was mainly segregated into the B4.1 posterior-vegetal blastomeres at the 8-cell stage. During the next stages, mitochondria was preferentially partitioned into cells of the B-line muscle and the A-line nerve cord precursor compared with each sister cell, endoderm in the 5th cleavage stage, and mesenchyme and notochord in the 6th cleavage stage. However, the mitochondria-rich cytoplasm is divided equally among the blastomeres of the animal hemisphere between the 8-cell and the 64-cell stages. When B6.2 blastomeres were isolated at the early 32-cell stage embryo and cultured in seawater, until control embryos reached the 64-cell stage, pattern of mitochondria distribution was similar to results of the coisolated B7.3 and B7.4 blastomeres from the 64-cell stage embryos. Therefore, it is likely that mitochondria are asymmetrically segregated into the marginal cells in the vegetal hemisphere of the ascidian embryo without cell-cell interaction.