A 11-year-old sexually intact female dog was presented because of a misshapen left elbow with a large mass. The masses on the elbow were diagnosed as high-grade canine mast cell tumor (cMCT) based on the cytological examination using fine-needle aspiration. Cytologically, the mass revealed moderate numbers of eosinophils, anisocytosis, anisokaryosis, and aberrant mitotic figures. The degree of cytologic dysplasia is diagnosed as moderate to severe. The blood analysis revealed low hematocrit and electrolytes. After examining the metastasis and surgical scope with radiographs and ultrasound, surgery was performed to removal the mass. Histopathologically, the mass revealed partially cytoplasmic granules, anisokaryosis of the nucleus, and mitotic counts. Scattered inflammatory cells and necrotic tissues were observed in the tumor. In this study, we found high-grade cMCT and treated the patient through a surgical approach.