The effects of the cation-to-anion resin ratio and bed depth on ion exchange performance of mixed-bed were studied at ultralow solution concentration. Breakthrough curves were experimentally obtained for NaCl solution as functions of resin ratio and bed depth. The bed depth affects the pattern of the sodium breakthrough curve but not the chloride breakthrough curve in beds because of the selectivity difference. Resin selectivity determines the shape of breakthrough curves. Some sodium and chloride breakthrough curves crossed at a point as a function of resin ratio. The lower cation-to-anion resin ratio showed the higher effluent concentration or treated volume of the crossover point regardless of the total resin weight.