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        검색결과 3

        1.
        2024.02 KCI 등재 SCOPUS 구독 인증기관 무료, 개인회원 유료
        Slipchip offers advantages such as high-throughout, low cost, and simple operation, and therefore, it is one of the technologies with the greatest potential for high-throughput, single-cell, and single-molecule analyses. Slipchip devices have achieved remarkable advances over the past decades, with its simplified molecular diagnostics gaining particular attention, especially during the COVID-19 pandemic and in various infectious diseases scenarios. Medical testing based on nucleic acid amplification in the Slipchip has become a promising alternative simple and rapid diagnostic tool in field situations. Herein, we present a comprehensive review of Slipchip device advances in molecular diagnostics, highlighting its use in digital recombinase polymerase amplification (RPA), loop-mediated isothermal amplification (LAMP), and polymerase chain reaction (PCR). Slipchip technology allows users to conduct reliable droplet transfers with high-throughput potential for single-cell and molecule analyses. This review explores the device’s versatility in miniaturized and rapid molecular diagnostics. A complete Slipchip device can be operated without special equipment or skilled handling, and provides high-throughput results in minimum settings. This review focuses on recent developments and Slipchip device challenges that need to be addressed for further advancements in microfluidics technology.
        4,000원
        2.
        2019.04 구독 인증기관·개인회원 무료
        The disadvantage of the current Red imported fire ants (RIFA) diagnostic is that it takes at least two days, because it is subjected to PCR amplification and DNA sequence identification process using an arthropod universal mitochondrial cytochrome oxidase I prime pair after genomic DNA extraction. In order to overcome this drawback, two RIFA specific genes were confirmed and used to develop techniques for identifying the species using various PCR methods. The conventional PCR method can be confirmed by the presence of amplified DNA, but additional time is required to confirm whether the PCR products are present or not. In contrast, SYBR green Real Time (RT) PCR or TaqMan probe RT PCR method has the advantage of confirming the results immediately after the reaction is completed. The new molecular diagnostic method has the advantage of shortening the time of two days, which is the biggest disadvantage of RIFA molecular diagnostic method, to 3 hours or less.
        3.
        2019.03 구독 인증기관 무료, 개인회원 유료
        Recently, the importance of on-site detection of pathogens has drawn attention in the field of molecular diagnostics. Unlike in a laboratory environment, on-site detection of pathogens is performed under limited resources. In this study, we tried to optimize the experimental conditions for on-site detection of pathogens using a combination of ultra-fast convection polymerase chain reaction (cPCR), which does not require regular electricity, and nucleic acid lateral flow (NALF) immunoassay. Salmonella species was used as the model pathogen. DNA was amplified within 21 minutes (equivalent to 30 cycles of polymerase chain reaction) using ultra-fast cPCR, and the amplified DNA was detected within approximately 5 minutes using NALF immunoassay with nucleic acid detection (NAD) cassettes. In order to avoid false-positive results with NAD cassettes, we reduced the primer concentration or ultra-fast cPCR run time. For singleplex ultra-fast cPCR, the primer concentration needed to be lowered to 3 μM or the run time needed to be reduced to 14 minutes. For duplex ultra-fast cPCR, 2 μM of each primer set needed to be used or the run time needed to be reduced to 14 minutes. Under the conditions optimized in this study, the combination of ultra-fast cPCR and NALF immunoassay can be applied to on-site detection of pathogens. The combination can be easily applied to the detection of oral pathogens.
        4,000원