A series of tests was conducted for full-scale single-pylon asymmetric cable-stayed bridges using a system of multiple shaking tables. The 2-span bridge length was 28 m, and the pylon height was 10.2 m. 4 different base conditions were considered: the fixed condition, RB (rubber bearings), LRB (lead rubber bearings), and HDRB (high damping rubber bearings). Based on investigation of the seismic response, the accelerations and displacements in the axial direction of the isolated bridge were increased compared to non-isolated case. However, the strain of the pylon was decreased, because the major mode of the structure was changed to translation for the axial direction due to the dynamic mass. The response of the cable bridge could differ from the desired response according to the locations and characteristics of the seismic isolator. Therefore, caution is required in the design and prediction in regard to the location and behavior of the seismic isolator.