The present study investigated runoff characteristics of non-point pollutants and discharge load amount according to the land utilization in Yeinam river basin. The land utilization of target basin was divided into paddy field, dry field, forest, residential area and composition area. The study on the runoff characteristics of non-point pollutants by rainfall-runoff process showed that COD, SS and T-P had the first-flushing effect with relatively high concentration in early-stage of the rainfall-runoff process, but the T-P revealed similar runoff characteristics. Event Mean Concentration(EMC) of BOD and COD according to the land utilization revealed the range of 3.11～15.50mg/L and 3.37～33.42mg/L, and the highest concentration of EMC corresponding to BOD and COD was detected in the paddy field. The EMC of SS showed 1.7～305.02mg/L and it's highest concentration was found in the dry field. The EMC of T-N and T-P represented the highest concentration in the paddy field and dry field with range of 0.91～8.76mg/L and 0.02～0.44mg/L.