Nuclear inspection is necessary to verify nuclear activities. If North Korea takes denuclearization, North Korea’s nuclear materials should be verified through non-destructive testing and destructive testing for nuclear material production. Since destructive testing of all substances is impossible, nondestructive testing is essential. Most non-destructive tests are performed by measuring the energy of gamma rays, but the characteristics of nuclear fuel can be evaluated by measuring neutron sources when enclosed with thick shields and when shielding structures are difficult to remove. Before the neutron source evaluation of MAGNOX used by North Korea, the relative characteristics will be evaluated later by analyzing the burnup, enrichment, and cooling time of the spent nuclear fuels discharged from the domestic nuclear power plant. This study evaluated the source strength and major nuclides according to burnup for the WH17×17 nuclear fuel assembly. The depletion calculation was conducted using SCALE 6.2 ORIGEN, and 3.5wt% enrichment, 10, 20, 30, 40, 50, 60 MWd/kg burnup, and five years cooling time, the minimum requirement for transport specified in the notice of the Nuclear Safety Commission, was applied. Although the impact assessment on enrichment should be evaluated with MCNP Tally to consider the fission reaction of the generated neutrons, this study only evaluated the spontaneous fission and (a, n) reactions that occurred first because it only evaluates the burnup impact. As burnup increased, neutron generation increased, and most of the total neutron strength occurred through spontaneous fission from the 10 MWd/kg burnup step. The influence of Pu-240 nuclides was dominant in the 10 MWd/kg burnup step but most neutrons were generated in tiny amounts of Cm- 244 generated from 20 MWd/kg burnup. Since DPRK’s 5 MWe Yongbyon MAGNOX has very low burnup (about 0.7 MWd/kg), the primary neutron sources of 10 MWd/kg, Am-241 and Pu isotopes, especially Pu-240, are expected to be used as indicators for evaluating spent nuclear fuel characteristics. If only specific nuclides are evaluated as major neutron sources at lower burnup than those evaluated in this study, in that case, the accuracy of non-destructive testing can be improved. Additionally, the evaluation according to the enrichment and cooling time should be considered as well.