This study investigated the fumigant activity of phosphine (PH3) on 2 kinds of mealybug (Pseudococcus longispinus and P. orchidicola (Hemiptera: Pseudococcidae)) adults and nymphs. All of the two mealybugs adults showed higher LCT99 values than nymphs, and P. longispinus showed higher tolerance than P. orchidicola in a 12 L desiccator. The absorption of phosphine on 13 nursery plants showed 12.2~41.5% difference depending on the plant. All of the mealybugs treated with phosphine 2 mg/L in 0.5 m3 fumigation chamber for 4 h showed 100% fumigant activity, except P. longispinus adult (approximately 90% at bottom part). However, when the exposure time was increased to 24 h, all of them showed 100% mortality. In the treatment of 10 m3 container, the 24 h treatment of phosphine showed 100% mortality to P. longispinus and P. orchidicola adults and nymphs. In all the experiments, no phytotoxicity of phosphine observed on 13 plants until 1 month after treatment.

A combination using phosphine (PH3) and ethyl formate (EF) was performed to compensate for the disadventages (long exposure time and phytotoxicity) of a single substance. P. longisipinus was more susceptible to mixed phosphine and ethyl formate than P. orchidicola in 12 L dessicator. Mortality of mixed treatment was higher than aggregated mortalities that treated individually, so it indicated to have a synergic effect on each other. Ethyl formate was showed higher adsorption rate than phosphine on imported nursery plants and showed differences depending on the plant. P. longispinus and P. orchidicola was showed 100% mortality when phosphine and ethyl formate was treated as 1 g/m3 and 30 g/m3 for 4 hour in 0.5 m3 fumigation chamber and in 10 m3 container. Phytotoxicity was evaluated to mixture of phosphine and ethyl formate at 1 month after treatment.

본 연구는 딸기 ‘매향’ (Fragaria × ananassa Duch.cv. Maehyang)의 수경재배를 이용하여 모주의 생육과 자묘 생산량 증대를 위한 최적 배양액 공급 농도 구명을 위해 수행되었다. 딸기 모주를 2017년 3월 22일 코코피트가 충진된 딸기 재배용 포트(64 × 27 × 18cm)에 정식하였다. 배양액은 뿌리 활착을 위해 11일 동안 EC 수준을 0.6dS·m-1의 농도로 점적 테이프를 이용하여 공급하였다. 뿌리 활착 후, 딸기 모주의 EC를 각각 0.6, 1.2, 1.8dS·m-1의 수준으로 처리해주었다. 정식 후 100일 째에 딸기의 모주와 자묘의 생육 특성을 측정하였다. 모 주의 초장은 EC 0.6dS·m-1 처리에서 유의적으로 높았고, 모주의 크라운 직경은 EC 1.8dS·m-1 처리에서 유의성 있게 두꺼웠다. 지상부와 러너 생체중 및 건물중 모두 EC 0.6, 1.2dS·m-1 처리에서 유의적으로 높았다. 런너의 개수는 모든 처리구에서 유의적인 차이를 보이지 않았다. 자묘 수는 EC 1.2dS·m-1 처리에서 16.7개로 가장 높은 값을 나타냈다. 그러나 딸기 세 번째 자묘의 생체중 및 건물 중은 0.6dS·m-1 처리에서 가장 높았다. 딸기 자묘 수는 1.2dS·m-1 처리에서 가장 많이 생산되었지만, 육묘기간 동안 딸기의 묘소질 측면에서는 낮은 EC 수준이 긍정적 인 결과를 보였다. 결과적으로, ‘매향’ 딸기의 육묘기간 동안 수경 재배용 배양액의 EC 수준을 0.6dS·m-1로 공급하는 것이 모주의 생육 및 자묘 생산에 가장 효과적인 것으로 나타났다.

The current protocols of methyl bromide (MeBr) fumigation on imported nursery plants, which are seriously infested with quarantine mealy bugs and scales, could be reevaluated because there were no limited uses of loading ratio, packing types etc. Another emerging issues is that the workplace health issues on MeBr fumigation are related with poor ventilation etc as well as well-known phytotoxic damages on them. In previous report, we have evaluated the efficacies of ethyl formate (EF) and phosphine (PH3) on different types of mealybugs. Herein, the phytotoxic damages of EF and PH3 on Alocasia, Sansevieria, Cactaceae and Croton genera were evaluated in terms of chlorophyll contents, color changes etc. This study showed that EF and PH3 fumigation on imported plants could be applicable to replacement of MeBr with further confirmation trials.