본연구의 목적은 흰쥐의 임신 초기에 있어서 착상의 유발에 PAF의 관련여부를 PAF의 수용체 길항제인 BN-52021d의 작용과 비교하여 결정하기 위함이다. 임신초기 각일에 점증하는 용량의 BN-52021 (체중 200g당 10g 내지 1.25mg)이 근육내로 주사되었을때 10g내지 250g 용량에서는 대조구에 비하여 착상부위의 수 혹은 이것을 가진 흰쥐의 수에 대하여 현저한 영향을 미치지 아니하였으나 1.25mg투여 경우에는 현저히 감소된 효과를 나타

A fertilized oocyte can get the competence for implantation through cleavage and stage-specific gene expression. These are under the control of autonomous and exogenous regulators including physiological culture condition. Endogenous and exogenous growth factors are considered as critical regulators of cleaving embryos during travel the oviduct and uterus. In this study, an effort was made to evaluate comprehensively the quality of embryos for implantation, grown in media enriched with EGF and PAF. The study evaluated developmental rates on given time, blastulation and hatching rates, and adhesion rates. Developmental rates of blastocyst to the hatching stage were significantly high in PAF treated group compared to the control in a dose-dependent manner but not in EGF group. Implantation rates were significantly high both PAF and EGF in a dose-dependent manner. H7, a PKC inhibitor, blocked the process of hatching of the blastocysts but combined treatment of EGF and PAF enhanced the hatching and implantation of blastocsyts. Based on these results it is suggested that EGF and PAF support acquirement of implantation competence at blastocyst stage through a PKC pathway.

15종의 항어혈성 중국 약용식물의 추출물에 대하여 PAF 수용체 결합 억제활성을 검색한 결과 4종의 약용식물, Campsis grandiflora, Dalbergia odorifera, Zanthoxylum nitidum, Vaccaria segetalis에서 50% 이상의 강한 PAF 결합 저해 활성이 나타났다.