This study was undertaken with packed tofu products to serve as a basic source for sanitary control of tofu production by detecting spoilage bacteria in tofu from which are isolated, investigating heat-resistance and growth characteristics of spoilage bacteria. Isolated strains were confirmed as relevant strains in tofu spoilage, and Strain No. Tl, T2 show 92% probability to be Enterobacter amnigenus, and 96% to be Flavobacterium indologenes according to the result of identifying strains by using Vitek system. Both strains had high viability at 35℃, pH 6.5. In the heat-resistance test of isolated strains, Enterobacter amnigenus T1 was treated for 2 mins, the number remained 56.3% of the initial number at 60℃, 37.8% at 70℃, 34.0% at 80℃ and 22.2% at 90℃, and Flavobacterium indologenes T2 was treated for 2 mins, the number remained 74.8% of the initial number at 60℃, 65.7% at 70℃, 37.8% at 80℃ and 9.3% at 90℃.