This study is to make a comparison between the one liquid emulsion with and without fish oil containing higher omega-3 fatty acid in parenteral nutrition (PN) of newborns. Serum chemistry and clinical complications from Medical Records of 76 newborns who had received PN with either SMOFlipidⓇ or ClinOleicⓇ for at least 7 days in NICU at Chosun Univeristy Hospital from January 2011 to December 2014 were reviewed retrospectively. Serum chemistry revealed no significant difference between the two groups except for total bilirubin (TB), glucose, blood urea nitrate (BUN), creatine (Cr), and cholesterol, but they even were not clinically significant as their values were within almost normal range. In terms of the difference in clinical complications, the incidence of cholestasis was higher in newborns given ClinOleicⓇ. However, there was no statistically significance. In this study, no significant difference between ClinOleicⓇ and SMOFlipidⓇ in their clinical efficacy was proven. But, considering the advantage of omega-3 fatty acid in reduction of cholestasis and infection risk, more studies may be needed using lipid emulsions containing higher content of fish oil with high omega-3 fatty acid, and they may be better if done with larger groups.